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SN/T 4067-2014: Quarantine protocol for measles to entry-exit monkey
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4067-2014 | English | 289 |
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Quarantine protocol for measles to entry-exit monkey
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SN/T 4067-2014
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Basic data | Standard ID | SN/T 4067-2014 (SN/T4067-2014) | | Description (Translated English) | Quarantine protocol for measles to entry-exit monkey | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 11,144 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Quoted Standard | GB/T 6682; GB 19489; SN/T 2025 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the monkey Isolation of measles virus, virus neutralization test, real-time PCR test, the technical requirements of blocking antibody ELISA test. This standard applies to the entry and exit monkey measles virus quarantine. | SN/T 4067-2014: Quarantine protocol for measles to entry-exit monkey---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for measles to entry-exit monkey
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Technical specification for quarantine of entry and exit monkey measles virus
Released on.2014-11-19
2015-05-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Hainan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Heilongjiang Entry-Exit Inspection and Quarantine
Bureau, Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Xinjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China,
Guangdong Entry-Exit Inspection and Quarantine Bureau.
The main drafters of this standard. Li Dandan, Xu Yigang, Ai Jun, Ji Xincheng, Xu Weijia, Lin Zhixiong, Wu Shannan, Huang Jihui.
Technical specification for quarantine of entry and exit monkey measles virus
1 Scope
This standard specifies the pathogen isolation of monkey measles virus, virus neutralization test, real-time fluorescent quantitative PCR test, blocking ELISA antibody
Technical requirements for the test.
This standard applies to the quarantine of entry and exit monkey measles virus.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB 19489 General requirements for laboratory biosafety
SN/T 2025 Animal Quarantine Laboratory Biosafety Practices
3 Abbreviations
The following abbreviations apply to this document.
B95a cells. simian lymphoblasts
CPE. cytopathic effect
Ct value. the number of cycles experienced by the fluorescent signal reaching the set threshold (cyclethreshold)
ELISA. enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay)
MV. measles virus
TCID50. half tissue culture infection (tissuecultureinfectivedose)
4 preliminary diagnosis
For the introduction of the disease, see A.1, the initial diagnosis can be based on clinical diagnosis and pathological changes, see A.2, A.3, and the laboratory is needed for diagnosis.
diagnosis.
5 virus separation
5.1 Equipment and equipment
Inverted optical microscope, carbon dioxide incubator, refrigerator (-20 ° C, -70 ° C), 50 μL, 100 μL micro-adjustable pipette, sterile
96-well cell culture plate, sterile plastic dripper, sterile plastic centrifuge tube (1.5mL) or 5mL~10mL sterile glass test tube, sterile cotton
Swab.
5.2 Reagents and materials
5.2.1 Virus. The standard strain of MV virus. See the GB 19489 for the experimental environment and protection requirements.
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