|
US$289.00 · In stock
Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email.
SN/T 4055-2014: Detection method of norovirus in shellfish. Conventional RT-PCR and real-time RT-PCR
Status: Valid
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4055-2014 | English | 289 |
Add to Cart
|
3 days [Need to translate]
|
Detection method of norovirus in shellfish. Conventional RT-PCR and real-time RT-PCR
| Valid |
SN/T 4055-2014
|
PDF similar to SN/T 4055-2014
Basic data | Standard ID | SN/T 4055-2014 (SN/T4055-2014) | | Description (Translated English) | Detection method of norovirus in shellfish. Conventional RT-PCR and real-time RT-PCR | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 11,145 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Quoted Standard | GB/T 19495.2 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the norovirus in shellfish common RT-PCR and real-time RT-PCR detection method. This standard applies to such as virus detection in shellfish promise. | SN/T 4055-2014: Detection method of norovirus in shellfish. Conventional RT-PCR and real-time RT-PCR
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection method of norovirus in shellfish.Conventional RT-PCR and real-time RT-PCR
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Replace SN/T 1635-2005
Norovirus detection method in shellfish
Ordinary RT-PCR method and real
Time-fluorescent RT-PCR method
Conventional RT-PCR and real-time RT-PCR
Released on.2014-11-19
2015-05-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces SN/T 1635-2005 "Norwalk virus detection method in shellfish, ordinary RT-PCR method and real-time fluorescence RT-
PCR method.
Compared with SN/T 1635-2005, the main technical changes of this standard are as follows.
--- Modified the standard Chinese name;
--- Modified the virus name, which was modified from Norovirus to Norovirus.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Guangxi Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Shanghai Entry and Exit Inspection of the People's Republic of China
Epidemic situation.
The main drafters of this standard. Liu Junyi, Pan Liangwen, Li Xiang, Lu Rong, Wei Meiliang, Chen Lijun, Luo Zhaofei.
The previous versions of the standards replaced by this standard are.
---SN/T 1635-2005.
Norovirus detection method in shellfish
Ordinary RT-PCR method and real
Time-fluorescent RT-PCR method
1 Scope
This standard specifies the common RT-PCR and real-time fluorescent RT-PCR detection methods for Nobel virus in shellfish.
This standard applies to the detection of Norovirus in shellfish.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 19495.2 Technical requirements for testing laboratory of genetically modified products
3 Terms and definitions
The following terms and definitions apply to this document.
3.1
Real-time fluorescence RT-PCR real-timefluorescence RT-PCR
The real-time fluorescent RT-PCR method is based on conventional RT-PCR and a specific fluorescent probe is added. The probe is a
The oligonucleotide is labeled with a reporter group and a quenching fluorophore at each end. When the probe is intact, the fluorescent signal emitted by the reporter group is
Quenching group absorption; during PCR amplification, the 5'-3' exonuclease activity of the Taq enzyme degrades the probe, allowing the reporter group and the quenching fluorophore
Separation, so that the fluorescence monitoring system can receive the fluorescent signal, that is, every time a DNA strand is amplified, a fluorescent molecule is formed, and the fluorescence is realized.
The accumulation of light signals is fully synchronized with the formation of the PCR product.
3.2
Ct value cyclethreshold
The number of cycles experienced by the fluorescent signal in each reaction tube reaching the set domain value.
4 reagents
All experimental reagents were of analytical grade; unless otherwise stated, the experimental water was distilled or deionized water.
4.1 Norovirus positive specimens. provided by the designated unit of the General Administration of Quality Supervision, Inspection and Quarantine. -80 ° C low temperature refrigerator storage.
4.2 Glycine buffer. see A.1.1.
4.3 PEG8000 solution. see A.1.2.
4.4 50 x TAE buffer. see A.1.3.
4.5 Ethidium bromide solution (10 μg/μL). see A.1.4.
4.6 1.5% agarose gel containing 0.5 μg/mL ethidium bromide. see A.1.5.
4.7 10× loading buffer. see A.1.6.
Tips & Frequently Asked Questions:Question 1: How long will the true-PDF of SN/T 4055-2014_English be delivered?Answer: Upon your order, we will start to translate SN/T 4055-2014_English as soon as possible, and keep you informed of the progress. The lead time is typically 1 ~ 3 working days. The lengthier the document the longer the lead time. Question 2: Can I share the purchased PDF of SN/T 4055-2014_English with my colleagues?Answer: Yes. The purchased PDF of SN/T 4055-2014_English will be deemed to be sold to your employer/organization who actually pays for it, including your colleagues and your employer's intranet. Question 3: Does the price include tax/VAT?Answer: Yes. Our tax invoice, downloaded/delivered in 9 seconds, includes all tax/VAT and complies with 100+ countries' tax regulations (tax exempted in 100+ countries) -- See Avoidance of Double Taxation Agreements (DTAs): List of DTAs signed between Singapore and 100+ countriesQuestion 4: Do you accept my currency other than USD?Answer: Yes. If you need your currency to be printed on the invoice, please write an email to [email protected]. In 2 working-hours, we will create a special link for you to pay in any currencies. Otherwise, follow the normal steps: Add to Cart -- Checkout -- Select your currency to pay.
|