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SN/T 4054-2014 English PDF

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SN/T 4054-2014: Determination of yohimbin, tadalafil, sildenafil and vardenafil in health foods for export. LC-MS/MS method
Status: Valid
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SN/T 4054-2014English449 Add to Cart 4 days [Need to translate] Determination of yohimbin, tadalafil, sildenafil and vardenafil in health foods for export. LC-MS/MS method Valid SN/T 4054-2014

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Basic data

Standard ID SN/T 4054-2014 (SN/T4054-2014)
Description (Translated English) Determination of yohimbin, tadalafil, sildenafil and vardenafil in health foods for export. LC-MS/MS method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X04
Classification of International Standard 67.050
Word Count Estimation 17,151
Date of Issue 12/1/2014
Date of Implementation 5/1/2015
Quoted Standard GB/T 6682
Regulation (derived from) State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the health foods yohimbine, vardenafil, sildenafil, tadalafil liquid chromatography - mass spectrometry/mass spectrometry. This standard applies to tablets, capsules and oral health food in yohimbe, vardenafil, sildenafil, tadalafi

SN/T 4054-2014: Determination of yohimbin, tadalafil, sildenafil and vardenafil in health foods for export. LC-MS/MS method


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Determination of yohimbin, tadalafil, sildenafil and vardenafil in health foods for export. LC-MS/MS method People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Exporting health foods in Yohimbe, vardenafil, Determination of sildenafil and tadalafil Liquid chromatography-mass spectrometry/mass spectrometry Released on.2014-11-19 2015-05-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. Please note that some of the contents of this document may involve patents. The issuing organization of this document is not responsible for identifying these patents. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Hunan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Hunan Institute of Inspection and Quarantine Science and Technology, Hunan Normal University. The main drafters of this standard. Zhang Ying, Wang Meiling, Zhu Shaohua, Zhang Fan, Yan Hongfei, Fu Shanliang, Li Yongjun, Chen Bo. Exporting health foods in Yohimbe, vardenafil, Determination of sildenafil and tadalafil Liquid chromatography-mass spectrometry/mass spectrometry

1 Scope

This standard specifies the liquid chromatography-mass spectrometry/mass spectrometry method for yohimbine, vardenafil, sildenafil and tadalafil in health foods. This standard applies to the quantitative determination of yohimbine, vardenafil, sildenafil and tadalafil in tablets, capsules and oral liquid health foods. And confirmation.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article. Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 Analytical laboratory water specifications and test methods

3 Principle

The yohimbine, vardenafil, sildenafil and tadalafil in the tablets and capsule samples were ultrasonically extracted with methanol, and the extract was diluted and filtered; The oral solution was directly diluted and filtered, and determined by liquid chromatography-mass spectrometry/mass spectrometry, and quantified by an external standard method.

4 reagents and materials

Unless otherwise specified, all reagents are of analytical grade and water is the first grade water in accordance with GB/T 6682. 4.1 Methanol. Liquid chromatography grade. 4.2 Acetonitrile. liquid chromatography grade. 4.3 Acetic acid. excellent grade pure. 4.4 0.1% acetic acid solution. Take 1 mL of acetic acid, dilute with water and dilute to 1000 mL. 4.5 0.1% acetic acid-methanol mixed solution (1, volume ratio). Measure 100mL 0.1% acetic acid solution, add 100mL methanol, mix spare. 4.6 Yohimbin (CAS number. 146-48-5, molecular formula. C21H26N2O3) Standard. purity is greater than or equal to 99.0%. 4.7 tadalafil (CAS number. 171596-29-5, molecular formula. C22H19N3O4) standard. purity is greater than or equal to 99.0%. 4.8 sildenafil (CAS number. 139755-83-2, molecular formula. C22H30N6O4S) standard. purity is greater than or equal to 99.0%. 4.9 vardenafil (vardenafil, CAS No.. 224785-91-5, molecular formula. C23H32N6O4S·HCl) Standard. purity greater than or equal 99.0%. 4.10 Standard stock solution. accurately weigh the appropriate amount of the above standard, and prepare a standard stock solution with a concentration of 1.0 mg/mL with methanol. Liquid, stored at 4 ° C protected from light. 4.11 Mixing standard intermediate solution. Take the appropriate amount of the above standard stock solution, and mix it with methanol to make a mixed standard intermediate solution with a concentration of 10μg/mL. Liquid, stored at 4 ° C protected from light.

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