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PCR methods for detecting Wuchereria bancrofti and Brugia malayi in mosquitoes
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SN/T 4043-2014
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Basic data Standard ID | SN/T 4043-2014 (SN/T4043-2014) | Description (Translated English) | PCR methods for detecting Wuchereria bancrofti and Brugia malayi in mosquitoes | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | B16 | Classification of International Standard | 07.080 | Word Count Estimation | 7,742 | Date of Issue | 12/1/2014 | Date of Implementation | 5/1/2015 | Quoted Standard | GB 19489; WS 233; SN/T 1300; SN/T 1553; SN/T 1560; SN/T 1596; SN/T 1876 | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | Issuing agency(ies) | General Administration of Customs | Summary | This standard specifies the border crossings PCR detection method on mosquitoes carrying bancrofti and Brugia malayi, including sample testing procedures, test methods, and determines and reports. This standard applies to the inspection and quarantine ins |
SN/T 4043-2014: PCR methods for detecting Wuchereria bancrofti and Brugia malayi in mosquitoes---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Mosquitoes carry heartworm (bancrofti, Brugia malayi) of PCR detection method)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Mosquitoes carry heartworm (bancrofti, Brugia malayi) of
PCR detection method
Issued on. 2014-11-19
2015-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine, the People's Republic of China
Exit Inspection and Quarantine and the State of Gansu, Sichuan, People's Republic of China Exit Inspection and Quarantine.
Drafters of this standard. Tian Hong, Liu Lijuan, Liu Fang, high Yufeng, Chen Xiao Xiao, Zhang Hongbing.
Mosquitoes carry heartworm (bancrofti, Brugia malayi) of
PCR detection method
1 Scope
This standard specifies the border crossings PCR detection method on mosquitoes carrying bancrofti and Brugia malayi, including sample testing procedures to detect
Method, and determines and reports.
This standard applies to the inspection and quarantine institutions Mosquitoes carry bancrofti and Brugia malayi detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
WS233 Microbiological and Biomedical Laboratories Biosafety Common Criteria
SN/T 1300 frontier port monitoring procedures Mosquitoes
SN/T 1553 into the exit of surveillance for aircraft medical vectors
SN/T 1560 into the outward vessel monitoring procedures medical vectors
SN/T 1596 exit into the vehicle monitoring procedures medical vectors
SN/T 1876 medical vectors specimen collection, production and preservation regulations
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Filarial Filaria
Spread by blood-sucking arthropods a class of parasitic nematodes. Adult parasites in vertebrate definitive host of the lymphatic system, subcutaneous tissue, abdominal
Cavity, chest, etc.. In certain blood-sucking arthropods larvae intermediate host in vivo development. When these intermediate host vampire, mature infection
Larvae that is, since its beak escape through the skin invade the final host body develop into adults. Filarial parasites in the human body there are eight kinds of popular
Mainly Wuchereria bancrofti (Wuchereriabancrofti) and malayi (Brugiamalayi) based. Two similar patterns, are all parasites
Milky silky, smooth surface, male and female variant.
3.2
Mosquitoes and Mosquito
The main media bancrofti is Culex pipiens (Culexpipienspallens) and Culex quinquefasciatus (Cx.pipiensquinque-
fasciatus), the main medium of Malay filariasis Anopheles sinensis (Anophelessinensis) and Anopheles Anopheles (An.anthropophagus) and the like.
3.3
PCR technology polymerasechainreaction
PCR technique is a method of in vitro enzymatic synthesis of specific DNA fragments by denaturing high temperature, low temperature and optimum temperature annealing steps and other anti-extending
It should form a cycle, cycle, so that the purpose of DNA can be amplified quickly, with high specificity, high sensitivity, easy to operate, saving time, etc.
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