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SN/T 4029-2014 English PDF

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SN/T 4029-2014: Skin sensitization - Local lymph node assay(LLNA) for cosmetics
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PDF similar to SN/T 4029-2014


Standard similar to SN/T 4029-2014

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Basic data

Standard ID SN/T 4029-2014 (SN/T4029-2014)
Description (Translated English) Skin sensitization - Local lymph node assay(LLNA) for cosmetics
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 7,795
Date of Issue 12/1/2014
Date of Implementation 5/1/2015
Regulation (derived from) State-Quality-Inspection-accreditation [2014] 614
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the cosmetic skin Anaphylaxis - local lymph node test method principles, test methods and procedures. This standard applies to raw materials and cosmetic product safety toxicology test.

SN/T 4029-2014: Skin sensitization - Local lymph node assay(LLNA) for cosmetics

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Cosmetics, skin allergy test local lymph France) People's Republic of China Entry-Exit Inspection and Quarantine Standards Cosmetics, skin allergy test local lymph law Skinsensitization-Locallymphnodeassay (LLNA) forcosmetics Issued on. 2014-11-19 2015-05-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Guangdong CIQ. The main drafters of this standard. Xuchong Hui, Cheng Shujun, Jiao Hong, PAN Fang, Wenqiao Ling, Liu Huizhi, Li Miaozhen. Cosmetics, skin allergy test local lymph law

1 Scope

This standard specifies the cosmetic skin Anaphylaxis - local lymph node test method principles, test methods and procedures. This standard applies to raw materials and cosmetic product safety toxicology test.

2 Terms and definitions

The following terms and definitions apply to this document. 2.1 Allergic skin reaction skinsensitisation Skin test substance produced immunogenic response. 2.2 Stimulation index stimulationindex; SI Induced lymphocyte proliferation in experimental animals under the effect of potentially allergenic substances obtained ratio, used to evaluate the test substance sensitizing ability.

3 Test Fundamentals

Local lymph node test method (LLNA) is the basic principle site of contact sensitizing chemicals can induce cell growth within the lymph nodes Colonization. Proliferative response and dose chemicals (and allergen sensitization force) proportional to the objective can be obtained by a simple method, quantitative Sensitization test data. LLNA test sample by comparing the test group with the control group proliferation dose - response relationship to assess the proliferation status. Comparative test sample test group and the control group, the proliferation rate of the solvent, i.e. the stimulation index (SI), when the index is greater than or equal to 3 test sample As a potential skin allergen for further evaluation. The method of the LLNA method is detected by radiolabeled cell proliferation and BrdU-ELISA assay. You can also use other Toxicity testing means the end of the evaluation of cell proliferation, but must provide adequate justification and scientific basis, including full references and methodology description. Test Method 4 4.1 test sample The test sample may be a liquid and a solid. Usually the liquid test sample without dilution, can be used directly liquid; solid test sample should be used Moist solvent or dissolved. The choice of solvent soluble consider the maximum test concentration and the test substance to form a solution/suspension in order to facilitate use. The solvent is recommended in order of preference. acetone/olive oil (4/1 by volume), dimethylformamide, methyl ethyl ketone, propylene glycol and dimethylsulfoxide, such as a Prepare sufficient scientific evidence, any other solvent. In some cases it is necessary to increase clinical or commercialization of the solvent system used for the test substance Agent as an additional control. Particular attention should be to make the hydrophilic substance dispersed in a solvent system, so that both moist skin, and will not be lost immediately, But to avoid the use of only aqueous solvents. 4.2 test solution 4.2.1 phosphate buffered saline (PBS). 0.1mol/LPBS solution, 4 ℃ preservation.

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