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SN/T 4018-2014: Detection of the viable but non-culturable bacterial in foods for export
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 4018-2014 | English | 189 |
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Detection of the viable but non-culturable bacterial in foods for export
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SN/T 4018-2014
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Basic data | Standard ID | SN/T 4018-2014 (SN/T4018-2014) | | Description (Translated English) | Detection of the viable but non-culturable bacterial in foods for export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 7,798 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Quoted Standard | GB/T 6682; GB 15193.19 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the microscope counting method and the Central African food exports bacteria culturable state fluorescence. This standard applies to the export of food to the Central African state of bacterial culture test. | SN/T 4018-2014: Detection of the viable but non-culturable bacterial in foods for export---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Central African food exports culturable bacteria test status)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Central African state export food culturable bacteria test
Issued on. 2014-11-19
2015-05-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Jilin People's Republic of China Exit Inspection and Quarantine, Jilin Agricultural University.
The main drafters of this standard. Meng Qingfeng, Spacing Form, Li Ying, Song Yun war, increasing Meng, Gao Yun Hang, Xiao into Rui, Liu Yang, Cai Yang, Luo Yan Fei,
Wang Zhenguo, Liu Jinhua, WEI Chunyan.
Central African state export food culturable bacteria test
1 Scope
This standard specifies the microscope counting method and the Central African food exports bacteria culturable state fluorescence.
This standard applies to the export of food to the Central African state of bacterial culture test.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB 15193.19 induced mutation approach, teratogens and carcinogens
3 Abbreviations
The following abbreviations apply to this document.
PI. propidium iodide.
SYT09. cyanine green dye.
VBNC. live, but non-culturable state.
4 Equipment and Materials
4.1 inverted fluorescence microscope. the objective of 100 ×/1.25 oil lens. Meanwhile, the green fluorescence microscope with excitation filter block, which
The excitation filter wavelength of 510nm ~ 570nm, absorption (curb) filter wavelength of 490nm.
4.2 slides and cover slips. Both claim no autofluorescence, smooth surface, the thickness of 0.17mm, a length greater than 25mm.
4.3 microporous membrane. a diameter of 25mm, pore size 0.2μm, white, smooth surface, no significant autofluorescence, hydrophilic.
4.4 Ink. Black ink commercially available.
4.5 Mirror Oil. no self-luminous cedar oil.
4.6 fluorescent dyes. SYTO9 final concentration 6μmol/L, the absorption wavelength of 480nm, fluorescence wavelength of 610nm; PI final concentration
30μmol/L, the absorption wavelength of 488nm, emission wavelength 630nm.
5 test procedure
5.1 Sample source
Samples taken from the bacterial culture negative.
5.2 hydrophobic microporous membrane check
First diameter 25mm aperture 0.2μm microporous membrane is completely immersed in sterile saline 2min, then remove the filter placed no
Bacteria dish, smooth side up, careful observation of the membrane surface. If the membrane surface of part of the area without water mark, it means that the hydrophobic membrane, should be re-more
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