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SN/T 3989-2014: Quarantine protocol for acute bee paralysis of honey bees
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 3989-2014 | English | 289 |
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Quarantine protocol for acute bee paralysis of honey bees
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SN/T 3989-2014
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Basic data | Standard ID | SN/T 3989-2014 (SN/T3989-2014) | | Description (Translated English) | Quarantine protocol for acute bee paralysis of honey bees | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 11,119 | | Date of Issue | 12/1/2014 | | Date of Implementation | 5/1/2015 | | Quoted Standard | GB/T 18088 | | Regulation (derived from) | State-Quality-Inspection-accreditation [2014] 614 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the pathogen detection of acute bee paralysis disease, including pathogen transmission electron microscope, RT-PCR method, fluorescent RT-PCR method. This standard applies to acute bee paralysis disease quarantine. | SN/T 3989-2014: Quarantine protocol for acute bee paralysis of honey bees---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for acute bee paralysis of honey bees
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Bee acne quarantine technical specification
Released on.2014-11-19
2015-05-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Jilin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Jilin University, Fujian Province, entry and exit
Inspection and Quarantine Bureau.
The main drafters of this standard. Song Zhanyi, Feng Xin, Wang Zhenguo, Zheng Teng, Liu Jinhua, Meng Rizeng, Wei Chunyan, Meng Qingfeng, Xiao Chengrui, Wang Weili.
Bee acne quarantine technical specification
1 Scope
This standard specifies the pathogen detection of bee acute paralysis, including pathogen transmission electron microscopy, RT-PCR method, fluorescence
RT-PCR method.
This standard applies to the quarantine of bees acute paralysis.
2 Normative references
The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article.
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 18088 entry and exit animal quarantine sampling
3 on-site quarantine
3.1 Bee colony inspection
All bee colonies to be inspected were observed outside the box to observe whether the behavior of the bee colony was abnormal, whether there were abnormal corpses outside the box and those with residual wings.
The young bee crawls, etc., from which the suspected bee colony is picked out and further checked in the box.
3.2 In-box inspection
To observe the spleen, mainly to check whether there are any abnormal objects in the box, and whether the bees have abnormal behaviors and forms. Whether the cover is abnormal,
Whether the larvae on the spleen have pathological manifestations and whether there are dead larvae, whether the larvae or adult bees carry parasites.
3.3 Individual inspection
Check the bees in the beehive and the dead bees in front of the nest door, and pull out the midgut to check whether there are symptoms of the disease. If the capsule is full of honey juice, the midgut is milky white.
Loss of elasticity, accumulation of yellow-brown feces in the hindgut is suspected of acute paralysis of bees, and timely evacuation of suspected or suspected bee colonies
The field was sampled and sent to the laboratory for further diagnosis.
4 Laboratory diagnosis
4.1 Transmission electron microscope observation
4.1.1 Equipment
Transmission electron microscope (magnification. 300,000 times), bench-top frozen high-speed centrifuge, micro-adjustable pipette (100μL~1000μL,
20μL~200μL, 2μL~20μL, 0.5μL~10μL), water bath.
4.1.2 Materials and reagents
4.1.2.1 PBS.
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