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SN/T 3979-2014: Determination of penicillinase in milk and dairy products - Cylinder plate method
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Determination of penicillinase in milk and dairy products - Cylinder plate method
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SN/T 3979-2014
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Basic data | Standard ID | SN/T 3979-2014 (SN/T3979-2014) | | Description (Translated English) | Determination of penicillinase in milk and dairy products - Cylinder plate method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100 | | Word Count Estimation | 15,135 | | Date of Issue | 7/14/2014 | | Date of Implementation | 3/1/2015 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | State inspection certification [2014] No. 353 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method for determination of microbiological raw milk and its products to sulbactam as inhibitors of ��- lactamase (plate method). This standard applies to fresh milk, sour milk, milk powder, etc. ��- lactamase detection. | SN/T 3979-2014: Determination of penicillinase in milk and dairy products - Cylinder plate method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Milk and dairy products β- lactamase determination cylinder plate method)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of milk and dairy products in the β- lactamase
Plate method
Issued on. 2014-07-14
2015-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard by the National Certification and Accreditation Administration Committee.
This standard was drafted. Chongqing, People's Republic of China Exit Inspection and Quarantine, in Chongqing Import and Export Food Safety Engineering Technology
Heart, Chinese Academy of Inspection and Quarantine, Ningbo People's Republic of China Exit Inspection and Quarantine, Southwest University.
The main drafters of this standard. Li Ying Kwok, Xue Xiaojing, Li Ling, Jin Yong, Zhou Qing, Nie Fuping, Xiaojin Wen, Tian Shimin, Lixian Liang, Wang Guomin,
Tian Jin Hong, Long Hao again.
Determination of milk and dairy products in the β- lactamase
Plate method
1 Scope
This standard specifies the method for determination of microbiological raw milk and its products to sulbactam as inhibitors of β- lactamase (plate method).
This standard applies to fresh milk, sour milk, milk powder, etc. β- lactamase detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 PRINCIPLE OF THE METHOD
The method uses sensitive to penicillin-type drugs absolute standard strains, penicillin G was added to the sample as a control, the use of sulbactam
Specific inhibition of β- lactamase activity, the size of the zone of inhibition by comparing the added sulbactam sulbactam not added generated by the sample to room
Measuring the samples contain β- lactamases drugs.
4 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade water for the three water GB/T 6682 regulations.
4.1 standard strains. Micrococcus luteus (Micrococcusluteus) CMCC (B) 28001, passage number should not exceed 14 times.
4.2 skimmed milk or cow milk.
4.3 penicillin G (BenzylpenicilinPotassiumGtetrahydrate) Standard. purity of greater than or equal to 99%; CAS Number. 113-
98-4; molecular weight. 372.48; Formula C16H17KN2O4S.
Within 4.4 β- lactamase (Penicilinase) standard 1
). CAS Number. 9001-74-5.
1) β- lactamase was provided by SIGMA Company. This information is given for the convenience of users of this standard does not mean that the product identified
can. If other equivalent products have the same effect, you can use these equivalent products.
4.5 sulbactam (Sulbactam). purity. 99.4%; CAS Number. 68373-14-8; molecular weight. 233.24; Molecular formula.
C8H11NO5S.
4.6 0.1mol/LTris-HCl solution (pH7.0, containing 0.1% BSA). Press A.1 preparation.
4.7 phosphate buffer solution (pH6.0). Press A.2 preparation.
4.8 G penicillin standard solution. Press A.3 preparation.
Lactamase standard solution within 4.9 β-. Press A.4 preparation.
4.10 sulbactam solution. Press A.5 preparation.
4.11 nutrient agar. Press A.6 preparation.
4.12 Brain Heart Infusion medium. Press A.7 preparation.
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