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SN/T 3979-2014 English PDF

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SN/T 3979-2014: Determination of penicillinase in milk and dairy products - Cylinder plate method
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SN/T 3979-2014English389 Add to Cart 3 days [Need to translate] Determination of penicillinase in milk and dairy products - Cylinder plate method Valid SN/T 3979-2014

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Basic data

Standard ID SN/T 3979-2014 (SN/T3979-2014)
Description (Translated English) Determination of penicillinase in milk and dairy products - Cylinder plate method
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard X16
Classification of International Standard 67.100
Word Count Estimation 15,135
Date of Issue 7/14/2014
Date of Implementation 3/1/2015
Quoted Standard GB/T 6682
Regulation (derived from) State inspection certification [2014] No. 353
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the method for determination of microbiological raw milk and its products to sulbactam as inhibitors of ��- lactamase (plate method). This standard applies to fresh milk, sour milk, milk powder, etc. ��- lactamase detection.

SN/T 3979-2014: Determination of penicillinase in milk and dairy products - Cylinder plate method


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(Milk and dairy products β- lactamase determination cylinder plate method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Determination of milk and dairy products in the β- lactamase Plate method Issued on. 2014-07-14 2015-03-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Please note that some of the content of this document may involve patents. Distribution of this document Institutions do not assume the responsibility to identify these patents. This standard by the National Certification and Accreditation Administration Committee. This standard was drafted. Chongqing, People's Republic of China Exit Inspection and Quarantine, in Chongqing Import and Export Food Safety Engineering Technology Heart, Chinese Academy of Inspection and Quarantine, Ningbo People's Republic of China Exit Inspection and Quarantine, Southwest University. The main drafters of this standard. Li Ying Kwok, Xue Xiaojing, Li Ling, Jin Yong, Zhou Qing, Nie Fuping, Xiaojin Wen, Tian Shimin, Lixian Liang, Wang Guomin, Tian Jin Hong, Long Hao again. Determination of milk and dairy products in the β- lactamase Plate method

1 Scope

This standard specifies the method for determination of microbiological raw milk and its products to sulbactam as inhibitors of β- lactamase (plate method). This standard applies to fresh milk, sour milk, milk powder, etc. β- lactamase detection.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis

3 PRINCIPLE OF THE METHOD

The method uses sensitive to penicillin-type drugs absolute standard strains, penicillin G was added to the sample as a control, the use of sulbactam Specific inhibition of β- lactamase activity, the size of the zone of inhibition by comparing the added sulbactam sulbactam not added generated by the sample to room Measuring the samples contain β- lactamases drugs.

4 Reagents and materials

Unless otherwise specified, the reagents were of analytical grade water for the three water GB/T 6682 regulations. 4.1 standard strains. Micrococcus luteus (Micrococcusluteus) CMCC (B) 28001, passage number should not exceed 14 times. 4.2 skimmed milk or cow milk. 4.3 penicillin G (BenzylpenicilinPotassiumGtetrahydrate) Standard. purity of greater than or equal to 99%; CAS Number. 113- 98-4; molecular weight. 372.48; Formula C16H17KN2O4S. Within 4.4 β- lactamase (Penicilinase) standard 1 ). CAS Number. 9001-74-5. 1) β- lactamase was provided by SIGMA Company. This information is given for the convenience of users of this standard does not mean that the product identified can. If other equivalent products have the same effect, you can use these equivalent products. 4.5 sulbactam (Sulbactam). purity. 99.4%; CAS Number. 68373-14-8; molecular weight. 233.24; Molecular formula. C8H11NO5S. 4.6 0.1mol/LTris-HCl solution (pH7.0, containing 0.1% BSA). Press A.1 preparation. 4.7 phosphate buffer solution (pH6.0). Press A.2 preparation. 4.8 G penicillin standard solution. Press A.3 preparation. Lactamase standard solution within 4.9 β-. Press A.4 preparation. 4.10 sulbactam solution. Press A.5 preparation. 4.11 nutrient agar. Press A.6 preparation. 4.12 Brain Heart Infusion medium. Press A.7 preparation.

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