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SN/T 3959-2014 English PDF

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SN/T 3959-2014: Detection of genetically modified components in sugarbeet. Conventional PCR method and real time PCR methods
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PDF similar to SN/T 3959-2014


Standard similar to SN/T 3959-2014

GB/T 11761   SN/T 0803.6   GB 2635   

Basic data

Standard ID SN/T 3959-2014 (SN/T3959-2014)
Description (Translated English) Detection of genetically modified components in sugarbeet. Conventional PCR method and real time PCR methods
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B34
Classification of International Standard 65.020
Word Count Estimation 9,935
Date of Issue 4/9/2014
Date of Implementation 11/1/2014
Quoted Standard GB/T 6682; GB/T 19495.2
Regulation (derived from) State inspection certification (2014) 210
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the general PCR and real-time PCR method beet and processed products in GMO detection. This standard applies to processed products and sugar beet qualitative detection of Genetically Modified.

SN/T 3959-2014: Detection of genetically modified components in sugarbeet. Conventional PCR method and real time PCR methods


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Detection of genetically modified components in sugarbeet. Conventional PCR method and real time PCR methods People's Republic of China Entry-Exit Inspection and Quarantine Standards Genetically modified beets ordinary PCR detection Methods and real-time PCR method Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Shanghai Entry-Exit Inspection and Quarantine Bureau, Xiamen People's Republic of China Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Heilongjiang Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine. The main drafters. Zhang Shuya, Chen Shuangya, Lu Rong, Li Xiang, high-Qin, Liu Yueming, Yang Jie Lin, Luan Feng Xia, water Zhu Fang, Pan Liangwen. Genetically modified beets ordinary PCR detection Methods and real-time PCR method

1 Scope

This standard specifies the general PCR and real-time PCR method beet and processed products in GMO detection. This standard applies to processed products and sugar beet qualitative detection of Genetically Modified.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.2 technical requirements for GMO testing laboratories 3 Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this document. 3.1 Terms and Definitions 3.1.1 Transgenic transgene The species itself does not have derived from other species of functional DNA sequences, through biotechnology to feed the species Line expression, so that the species to obtain new varieties of features. 3.1.2 Real-time fluorescence PCR real-timefluorescentpolymerasechainreaction; RT-PCR Real time polymerase chain reaction. Means adding a fluorophore in a polymerase chain reaction system, real-time fluorescence signal accumulated Monitor the entire PCR process, the strength of the fluorescence signal directly reflects the number of templates. 3.1.3 Endogenous gene endogenousgene In the examined species constant copy number, gene allele variation is not displayed. The gene can be used to determine the species specificity. 3.1.4 Exogenous gene exogenousgene Use of other bio-genetic bioengineering technology transferred, so that the performance of the new biological species biological traits. 3.2 Acronyms Ct values. cyclethreshold, the number of cycles in each reaction tube fluorescent signal reaches the set threshold experienced. CTAB. cetyltrithylammoniumbromide, cetyl dimethyl ammonium bromide. EDTA. ethylenediaminetetraaceticacid, ethylenediaminetetraacetic acid. Tris. tris (hydroxymethyl) aminomethane, tris (hydroxymethyl) aminomethane.

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