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SN/T 3957-2014 English PDF

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SN/T 3957-2014: Identification of Cordyceps sinensis(Berk.)Sacc. - Real-time PCR
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PDF similar to SN/T 3957-2014


Standard similar to SN/T 3957-2014

GB/T 41907   SN/T 4806   SN/T 4527   SN/T 4458   

Basic data

Standard ID SN/T 3957-2014 (SN/T3957-2014)
Description (Translated English) Identification of Cordyceps sinensis(Berk.)Sacc. - Real-time PCR
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C23
Classification of International Standard 11.120.10
Word Count Estimation 8,840
Date of Issue 4/9/2014
Date of Implementation 11/1/2014
Quoted Standard GB/T 6682; GB/T 27403; SN/T 1193
Regulation (derived from) State inspection certification (2014) 210
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the authenticity of identification of Cordyceps real-time PCR method. This standard applies to Cordyceps [Cordyceps sinensis (Berk.) Sacc.] Real-time PCR for identification of natural raw materials and fermentation mycelium.

SN/T 3957-2014: Identification of Cordyceps sinensis(Berk.)Sacc. - Real-time PCR

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Cordyceps identify the authenticity of real-time PCR method) People's Republic of China Entry-Exit Inspection and Quarantine Standards Cordyceps authenticity of identification method for real-time PCR IdentificationofCordycepssinensis (Berk.) Sacc.-Real-timePCR Issued on. 2014-04-09 2014-11-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Guangdong CIQ. The main drafters of this standard. Ma Jun, Xie force, Hu learning difficulties, Chen Yuan tree, Zhang Jun, Liu Jin, slightly east. Cordyceps authenticity of identification method for real-time PCR

1 Scope

This standard specifies the authenticity of identification of Cordyceps real-time PCR method. This standard applies to Cordyceps [Cordycepssinensis (Berk.) Sacc.] Real-time fluorescence of natural raw materials and fermentation mycelium PCR identification.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 27403 laboratory quality control of food molecular biology standardized testing SN/T 1193 genetic testing laboratory technical requirements 3 Terms, definitions and abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this document. Ct values cyclethreshold The number of cycles when fluorescence signal of each reaction tube reaches a set threshold experienced. 3.2 Acronyms The following abbreviations apply to this document. Tris. tris (hydroxymethyl) aminomethane, tris (hydroxymethyl) aminomethane EDTA. ethylenediaminetetraaceticacid, ethylenediaminetetraacetic acid CTAB. cetyltrithylammoniumbromide, cetyl trimethyl ammonium bromide PCR. polymerasechainreaction, polymerase chain reaction DNA. deoxyribonucleicacid, DNA dNTP. deoxyribonucleosidetriphosphate, deoxynucleotide triphosphates dATP. deoxyadenosinetriphosphate, deoxyadenosine triphosphate dCTP. deoxycytidinetriphosphate, deoxycytidine triphosphate dGTP. deoxyguanosinetriphosphate, deoxyguanosine triphosphate dTTP. deoxythymidinetriphosphate, deoxythymidine triphosphate Taq. Thermusaquaticus, aquatic Thermus OD. opticaldensity, optical density

4 Reagents and materials

Unless otherwise specified, all reagents were of analytical grade or biochemical reagents. Test water line with GB/T 6682 requirements. All reagents were

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