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SN/T 3924-2014: Test method for the enumeration of coliform and fecal coliform in shellfish for export
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 3924-2014 | English | 259 |
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Test method for the enumeration of coliform and fecal coliform in shellfish for export
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SN/T 3924-2014
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Basic data | Standard ID | SN/T 3924-2014 (SN/T3924-2014) | | Description (Translated English) | Test method for the enumeration of coliform and fecal coliform in shellfish for export | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 10,183 | | Date of Issue | 4/9/2014 | | Date of Implementation | 11/1/2014 | | Quoted Standard | GB/T 4789.20; GB 17378.3; SN/T 1538.1; SN/T 1538.2 | | Regulation (derived from) | State inspection certification (2014) 210 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the shellfish coliform, fecal coliform count method. This standard applies to bivalve molluscs, fresh shucked shellfish meat, fresh shucked and frozen shellfish meat frozen half-shell shellfish coliform, fecal coliform count. | SN/T 3924-2014: Test method for the enumeration of coliform and fecal coliform in shellfish for export
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Test method for the enumeration of coliform and fecal coliform in shellfish for export
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Export shellfish coliform, fecal coliform
Detection method
Issued on. 2014-04-09
2014-11-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Fujian Exit Inspection and Quarantine.
The main drafters of this standard. Chen Bin, Zheng Jing, Chen Junyu, Chen Shuyi, Xue Zhimin, Huang Xiaorong, Zheng Linyi, Dong Jian, Lin Jie, Peng Huayi.
Export shellfish coliform, fecal coliform
Detection method
1 Scope
This standard specifies the shellfish coliform, fecal coliform count method.
This standard applies to bivalve molluscs, fresh shucked shellfish meat, fresh shucked and frozen shellfish meat frozen half-shell shellfish coliform, fecal coliform
Group count.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 4789.20 microbiological examination of food hygiene inspection of aquatic food
GB 17378.3 specification for marine monitoring Part 3. Sample collection, storage and transportation
SN/T 1538.1 media preparation Guide - Part 1. General laboratory quality assurance prepared medium
SN/T 1538.2 media preparation Guide Part 2. A Practical Guide to medium performance test
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Coliform coliforms
Aerobic and facultative anaerobic, can break down lactose at 36 ℃ 48h produce acid and gas group of gram-negative bacillus free.
3.2
Fecal coliform fecalcoliforms
Aerobic and facultative anaerobic, capable of 44.5 ℃ ± 0.5 ℃, 24h ferment lactose produce acid and gas group of gram-negative bacillus without the
Heat-resistant coliform bacteria can be called (thermotolerantcoliformorganisms).
4 test methods
4.1 Principle
The most likely approximation (MPN) method is a statistical combination of microbiology and a quantitative assay. Samples were serially diluted and tested training
After the raise, according to the lowest dilution of its outstanding growth and the growth of the highest dilution, using statistical probability theory deduced coliform, fecal coliform
The maximum number of possible group in the test sample.
4.2 media and reagents
4.2.1 lauryl sulfate tryptone (LST) broth. See Appendix A A.2.
4.2.2 lactose (LB) broth. See A.3.
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