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SN/T 3897-2014: Determination of tetracycline antibiotics in cosmetics
Status: Valid
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| SN/T 3897-2014 | English | 419 |
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Determination of tetracycline antibiotics in cosmetics
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SN/T 3897-2014
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Basic data | Standard ID | SN/T 3897-2014 (SN/T3897-2014) | | Description (Translated English) | Determination of tetracycline antibiotics in cosmetics | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 16,111 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | AQSIQ notification issued in 2014 on the first batch of 230 industry-standard entry-exit inspection and quarantine | | Issuing agency(ies) | General Administration of Customs | | Summary | This Standard specifies the cosmetics minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline, methylene oxytetracycline, doxycycline, tetracycline difference to dehydration, dehydration tetracycline nine kinds of tetracyclines deter | SN/T 3897-2014: Determination of tetracycline antibiotics in cosmetics---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Cosmetics tetracyclines - Determination)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Determination of cosmetics tetracycline antibiotics
Issued on. 2014-01-13
2014-08-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters of this standard. Huang Chao-chun, Xie Wen, Hou Jianbo Shi Ying Chu, Chen Lingling, F. Cheng Jie, handsome big Yao, Sun Chengqi.
Determination of cosmetics tetracycline antibiotics
1 Scope
This standard specifies the cosmetics minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline, oxytetracycline armor, strong mildew
Su, tetracycline difference to dehydration, dehydration nine tetracycline tetracyclines measured by high performance liquid chromatography (HPLC) and liquid chromatography - mass
/ Mass Spectrometry (LC-MS/MS) detection.
This standard applies to the determination of toner, lotion, mask, soap, facial cleanser, acne cream of 9 tetracycline antibiotics.
The standard liquid chromatography - mass spectrometry/mass spectrometry for the arbitration law.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
The first method HPLC
3 Method summary
Methanol - liquid chromatograph oxalic acid solution as solvent, ultrasonic extraction, centrifugation, filtration, equipped with a diode array detector and external standard
Statutory amount.
4 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade, the water is a water line with GB/T 6682 regulations.
4.1 acetonitrile. liquid chromatography.
4.2 methanol. liquid chromatography.
4.3 oxalic acid. pure class distinctions.
4.4 formic acid. liquid chromatography.
4.5 ammonia.
4.6 phosphoric acid. pure class distinctions.
4.7 0.2% phosphoric acid solution. Pipette 2.0mL phosphoric acid (4.6), water volume to 1000mL.
4.8 0.02mol/L oxalic acid solution. Weigh 0.90g oxalic acid (4.3), dissolved in water and dilute to 500mL.
4.9 Methanol - oxalic acid solution (11 volume). Measure 500mL of methanol (4.2) and 500mL solution of oxalic acid (4.8) were mixed.
4.10 minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline, methylene oxytetracycline, doxycycline, tetracycline difference to dehydration, de
Water tetracycline standards. purity of greater than or equal to 90%, see appendix A in Table A.1.
4.11 standard stock solution. the right amount of conversion were referred learn standard (4.10) were dissolved in methanol and set the volume, the solution concentration
200μg/mL, the standard stock solution stored below -18 ℃, valid for six months.
4.12 intermediate mixed standard solution. accurate Pipette 5.0mL respectively minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline,
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