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Real-time fluorescence PCR method for detection of genetically modified components in triffid flax(Event FP967)
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SN/T 3895-2014
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Basic data Standard ID | SN/T 3895-2014 (SN/T3895-2014) | Description (Translated English) | Real-time fluorescence PCR method for detection of genetically modified components in triffid flax(Event FP967) | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | B04 | Classification of International Standard | 65.020 | Word Count Estimation | 12,138 | Quoted Standard | GB/T 6682; GB/T 19495.2; GB/T 19495.3 | Regulation (derived from) | AQSIQ notification issued in 2014 on the first batch of 230 industry-standard entry-exit inspection and quarantine | Issuing agency(ies) | General Administration of Customs | Summary | This standard specifies the transgenic linseed FP967 lines (also known as CDC Triffid) real-time PCR detection method. This standard applies to transgenic linseed FP967 lines (also known as CDC Triffid) qualitative detection. |
SN/T 3895-2014: Real-time fluorescence PCR method for detection of genetically modified components in triffid flax(Event FP967) ---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(Flaxseed FP967 transgenic strains of real-time PCR detection method)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Transgenic Linseed FP967 line real-time fluorescence
PCR detection method
componentsintriffidflax (EventFP967)
Issued on. 2014-01-13
2014-08-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
The standard reference of the EU-CRL "NOST-Specconstruct-specificmethodforthedetectionofCDCtriffid
flax (EventFP967) usingreal-timePCR "method.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Liaoning Province Exit Inspection and Quarantine.
The main drafters of this standard. Juan Cao occasion, Zhang Ying, Wang Jinling, Xu Junyi, Jia Jinsheng.
Transgenic Linseed FP967 line real-time fluorescence
PCR detection method
1 Scope
This standard specifies the transgenic linseed FP967 lines (also known as CDCTriffid) real-time PCR detection method.
This standard applies to transgenic linseed FP967 lines (also referred to CDCTriffid) for qualitative detection.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 19495.2 technical requirements for GMO testing laboratories
GB/T 19495.3 GMO detection of nucleic acid extraction and purification method
3 Terms, definitions and abbreviations
3.1 Terms and Definitions
The following terms and definitions apply to this document.
3.1.1
Transgenic transgene
The species itself does not have derived from other species of functional DNA sequences, by introducing a variety of means to make it into the species
Line expression, in order to obtain new varieties of the species characteristics.
3.1.2
Endogenous gene endogenousgene
Species cultivated in copy number constant, allele variation is not displayed. The gene can be used for a purpose genome
Analysis of the gene.
3.2 Acronyms
The following abbreviations apply to this document.
Ct values. C. Cycle, t. threshold, the number of cycles in each reaction tube fluorescent signal reaches the set threshold experienced.
DNA. deoxyribonucleicacid, DNA.
dNTP. deoxyribonucleosidetriphosphate, deoxynucleoside triphosphates.
EDTA. ethylenediaminetetraaceticacid, ethylenediaminetetraacetic acid.
NOST-Spec. NOSterminatorandspectinomycinresistancegene, NOS terminator - spectinomycin resistance gene
because. Spectinomycin resistance gene strains of transgenic linseed FP967 is a unique gene, it is not currently in any other genetically modified crops
use.
PCR. polymerasechainreaction, called PCR.
SAD. stearoyl-acylcarrierproteindesaturase, stearoyl - acyl carrier protein desaturase.
Tris. tris (hydroxymethyl) aminomethane, tris (hydroxymethyl) aminomethane.
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