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SN/T 3868-2014: Determination of aflatoxin B1, B2, G1, G2 in edible oil for export - Immunoaffinity column clean-up and HPLC with fluorescence detection
Status: Valid
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Determination of aflatoxin B1, B2, G1, G2 in edible oil for export - Immunoaffinity column clean-up and HPLC with fluorescence detection
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SN/T 3868-2014
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Basic data | Standard ID | SN/T 3868-2014 (SN/T3868-2014) | | Description (Translated English) | Determination of aflatoxin B1, B2, G1, G2 in edible oil for export - Immunoaffinity column clean-up and HPLC with fluorescence detection | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X14 | | Classification of International Standard | 67.200.10 | | Word Count Estimation | 12,175 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | AQSIQ notification issued in 2014 on the first batch of 230 industry-standard entry-exit inspection and quarantine | | Issuing agency(ies) | General Administration of Customs | | Summary | This Standard specifies the vegetable export of aflatoxin B1, B2, G1, G2 detection methods. This Standard applies to peanut oil, olive oil, sesame aflatoxin testing. | SN/T 3868-2014: Determination of aflatoxin B1, B2, G1, G2 in edible oil for export - Immunoaffinity column clean-up and HPLC with fluorescence detection
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(Vegetable export of aflatoxin B1; B2; G1; G2- detection - immunoaffinity column HPLC purification)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Vegetable oil exports aflatoxins B1, B2, G1, G2 of
Detection immunoaffinity column purification by HPLC
DeterminationofaflatoxinB1, B2, G1, G2inedibleoilforexport-
Issued on. 2014-01-13
2014-08-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Shandong CIQ is responsible for drafting.
The main drafters of this standard. Bao Lei, Xu Yanli, Wu Zhenxing, Jing Ping, Luehning, Jin Ying.
Vegetable oil exports aflatoxins B1, B2, G1, G2 of
Detection immunoaffinity column purification by HPLC
1 Scope
This standard specifies the vegetable export of aflatoxin B1, B2, G1, G2 detection methods.
This standard applies to peanut oil, olive oil, sesame aflatoxin testing.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Method summary
Aflatoxins in the sample with methanol - water (5545, volume ratio) extract, the extract was centrifuged, filtered, diluted with immunoaffinity column
Purification by liquid chromatography with fluorescence detector post-column derivatization method for the determination of aflatoxin toxin content, external standard.
4 Reagents and materials
Unless otherwise specified, the use of chemical reagents were of analytical grade water as a water GB/T 6682 regulations.
4.1 Methanol. HPLC grade.
4.2 acetonitrile. HPLC grade.
4.3 nitric acid. 65%.
4.4 sodium chloride.
4.5 potassium bromide.
Extract 4.6. methanol - water (5,545, volume ratio).
4.7 Eluent. methanol - water (1,090, volume ratio).
4.8 aflatoxin standard. A6636 (aflatoxin B1), A9887 (aflatoxin B2), A0138 (aflatoxin G1),
A0263 (aflatoxin G2), SigmaAldrich, or equivalent person; purity ≥99.0%.
4.9 aflatoxin standard stock solution. the 1mg aflatoxin standards B1, B2, G1 and G2 (4.8) were dissolved in acetonitrile (4.2),
And set the volume to 10mL, was 100μg/mL standard stock solution Ⅰ, accurate pipetting four kinds of aflatoxin standard stock solution Ⅰ each 1mL, points
Do not dissolved in acetonitrile (4.2) and set the volume to 10mL, was 10μg/mL aflatoxin B1, B2, G1, G2 standard stock solution Ⅱ, save in
-18 ℃.
4.10 aflatoxin mixed intermediate standard solution. accurate Pipette appropriate amount of four kinds of 10μg/mL stock solution of aflatoxin standard Ⅱ, placed in the same
A flask with acetonitrile (4.2) diluted to the mark, as the standard intermediate mixed solution containing total aflatoxins 400ng/mL (containing
Aflatoxin B1, B2, G1, G2, respectively 200,50,100 and 50ng/mL), stored at -18 ℃.
4.11 aflatoxin standard working solution. Pipette the appropriate amount of aflatoxin mixing different volumes of intermediate standard solution (4.10), 50% formamide
Alcohol - Aqueous formulation according to Table 1 the concentration gradient of the standard curve, use namely distribution.
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