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SN/T 3395-2012: PCR detection methods for tick borne encephalitis virus, borrelia burgdorferi, coxiella burneti
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 3395-2012 | English | 239 |
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PCR detection methods for tick borne encephalitis virus, borrelia burgdorferi, coxiella burneti
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SN/T 3395-2012
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Standard similar to SN/T 3395-2012 SN/T 5268 Basic data | Standard ID | SN/T 3395-2012 (SN/T3395-2012) | | Description (Translated English) | PCR detection methods for tick borne encephalitis virus, borrelia burgdorferi, coxiella burneti | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 9,953 | | Quoted Standard | GB 19489; SN/T 1293; WS 233 | | Regulation (derived from) | National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the border port of ticks carry pathogens in tick-borne encephalitis virus, Borrelia burgdorferi, Coxiella burnetii PCR assay. This standard applies to the border port of ticks carry important pathogen (tick-borne encephalitis virus | SN/T 3395-2012: PCR detection methods for tick borne encephalitis virus, borrelia burgdorferi, coxiella burneti
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
PCR detection methods for tick borne encephalitis virus, borrelia burgdorferi, coxiella burneti
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Ticks carry the tick-borne encephalitis virus, Borrelia burgdorferi,
Coxiella burnetii PCR detection method
PCRdetectionmethodsfortickborneencephalitisvirus, borrelia
burgdorferi, coxielaburneti
Issued on. 2012-12-12
2013-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, Heilongjiang People's Republic of China Exit Inspection and Quarantine, the Chinese people
Republic of Liaoning CIQ.
The main drafters of this standard. Yang Yu, Han Hui, Chi Ying, Li, Song Feng Lin, Wang Jing, Hu new holes, including force, Yao Doe, Wei Lin, Wang Qin Zhong,
Liu Feng, Sun Xiaohong.
Ticks carry the tick-borne encephalitis virus, Borrelia burgdorferi,
Coxiella burnetii PCR detection method
1 Scope
This standard specifies the border crossings ticks carrying the pathogen in tick-borne encephalitis virus, Borrelia burgdorferi, Coxiella burnetii PCR detection method.
This standard applies to border crossings ticks carry important pathogens (tick-borne encephalitis virus, Borrelia burgdorferi, Coxiella burnetii) investigation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB 19489 General requirements for laboratory biosafety
SN/T 1293 ticks frontier port monitoring procedures
WS233 laboratory bio-safety grade Ⅱ Laboratory requirements
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Tick-borne encephalitis virus tickborneencephalitisvirus
It belongs to the Flaviviridae Flavivirus capsid icosahedral symmetry outer capsule, containing hemagglutinin glycoprotein, a single-stranded nucleic acid Underlying RNA, viral
In nature cycle in ticks and wild animals, rodents such as squirrels, voles, hedgehogs etc based disease source of infection. When sucking ticks infected rodents
After the blood of animals, in vivo propagation of the virus in ticks, and wintering and passage through eggs, so the tick is not only the media is an important reservoir host.
3.2
Borrelia burgdorferi Borreliaburgdorferi
Belonging to the genus Borrelia, the causative agent of Lyme disease is caused by Gram-negative, genome consists of a linear chromosome and a plurality of annular
And linear plasmids, the media mainly some hard ticks, the main reservoir hosts are voles and mammals.
3.3
Coxiella burnetii Coxiellaburneti
Belonging to the genus Coxiella, Q fever is caused by zoonotic pathogens, Gram-negative, transmitted by ticks to wild rodents
And livestock, and then subjected to dye animal feces, urine pollution of the environment by human contact or respiratory infections.
4 bio-safety requirements
Tick-borne pathogen detection should biosafety laboratory grade Ⅱ (BSL-2) were within the laboratory operations in accordance with GB 19489,
WS233 requirements.
5 experimental equipment
PCR amplification; biological safety cabinet; electrophoresis; refrigerator; ordinary desktop centrifuge; speed refrigerated centrifuge (speed up to 20000g);
Gel imaging system; micro-adjustable pipette (10μL, 100μL, 1000μL); vortex shaker; clean bench.
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