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SN/T 3380-2012: Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export. ELISA method
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Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export. ELISA method
| Valid |
SN/T 3380-2012
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Standard similar to SN/T 3380-2012 SN/T 5268 Basic data | Standard ID | SN/T 3380-2012 (SN/T3380-2012) | | Description (Translated English) | Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export. ELISA method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 8,881 | | Date of Issue | 12/12/2012 | | Date of Implementation | 7/1/2013 | | Regulation (derived from) | State-Quality-Inspection-accreditation (2012) 777 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the method of enzyme-linked immunoassay four nitrofuran metabolite residues in food of animal origin exports. This standard applies to chicken, pork, crayfish, back to the fish, milk, honey and other food of animal origin furazolid | SN/T 3380-2012: Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export. ELISA method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of residues of nitrofuran metabolites in foodstuffs of animal origin for export.ELISA method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Export of food of animal origin nitrofuran metabolites
Determination of the residual ELISA
Issued on. 2012-12-12
2013-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Institutions do not assume the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Jiangsu Entry-Exit Inspection and Quarantine Bureau, Changzhou, People's Republic of China Entry-Exit Inspection and Quarantine
Bureau, People's Republic of China Shandong Entry-Exit Inspection and Quarantine Yancheng People's Republic of China.
The main drafters of this standard. Yu Bing, Cai Baoliang, Li Dongming, Yuan Tao, Yuan Fang, Xu Xing help, DingTao.
Export of food of animal origin nitrofuran metabolites
Determination of the residual ELISA
1 Scope
This standard specifies four methods of enzyme-linked immunosorbent assay nitrofuran metabolites residues in food of animal origin exports.
This standard applies to chicken, pork, crawfish, back to the fish, milk, honey and other food of animal origin furazolidone metabolite (AOZ), furan
It is one metabolite (AMOZ), nitrofurazone metabolite (SEM), nitrofurantoin metabolite (AHD) residues detected.
Principle 2
Determination of the basis of this method is a competitive enzyme immunoassay, the overall process includes four nitrofuran metabolites residues detected. in
Microtiter plates pre-coated strips and conjugate antigen in the sample nitrofuran metabolites residues after derivatized and even pre-coated on the strips are of
Associated antigen antibody anti-competitive corresponding derivatives of nitrofuran metabolites after adding enzyme-labeled secondary antibody with TMB chromogenic substrate, the sample absorbance value
Its content contained nitrofuran metabolites residues negatively correlated with the standard curve and multiplied by the corresponding dilution factor can be derived sample
The remaining amount of nitrofuran metabolites residues.
3 Reagents and materials
Unless otherwise specified, the reagents were of analytical grade, water is deionized water.
3.1 methanol.
3.2 ethyl acetate.
3.3 n-hexane.
3.4 acetonitrile.
3.5 of concentrated hydrochloric acid.
3.6 sodium hydroxide.
3.7 dipotassium hydrogen phosphate.
3.8 nitroso ferricyanide.
3.9 zinc sulfate.
3.10 pairs carboxybenzaldehyde.
3.11 1mol/L hydrochloric acid (HCl). Take 8.6mL of concentrated HCl and add water to dissolve 100mL.
3.12 0.1mol/L potassium phosphate dibasic (K2HPO4). said 22.8gK2HPO4 · 3H2O dissolved with deionized water solution to 1L.
3.13 nitroso ferricyanide (K2Fe (CN) 5 · NO · 3H2O) solution. 12.5g nitroso ferricyanide deionized water to dissolve
100mL.
3.14 zinc sulfate (ZnSO4 · 7H2O) solution. 29.8g zinc sulfate dissolved with deionized water to dissolve 100mL.
3.15 derivatization reagent (0.01mol/L CARBOXYBENZALDEHYDE solution). 15.013mg CARBOXYBENZALDEHYDE dissolved in methanol and set the volume to
10mL.
3.16 Standard. includes AOZ, AMOZ, SEM and AHD four standards, the purity of ≥99%.
Preparation of standard solution of 3.17. were accurately weighed amount of AOZ, AMOZ, SEM and AHD four standards, paired with acetonitrile
1mg/mL standard stock solution at 4 ℃ ~ 8 ℃ preservation conditions.
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