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SN/T 3328-2012: Protocol of identification of scallops. Simple-sequence repeats (SSR)
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 3328-2012 | English | 209 |
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Protocol of identification of scallops. Simple-sequence repeats (SSR)
| Valid |
SN/T 3328-2012
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Standard similar to SN/T 3328-2012 SN/T 5268 Basic data | Standard ID | SN/T 3328-2012 (SN/T3328-2012) | | Description (Translated English) | Protocol of identification of scallops. Simple-sequence repeats (SSR) | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Word Count Estimation | 8,851 | | Quoted Standard | GB/T 6682; SN/T 1193 | | Regulation (derived from) | National Quality Inspection (2012) 777; industry standard filing Notice 2013 No. 4 (No. 160 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the scallop species SSR identification. This standard applies to inbound and outbound chilled, dried, cooked canned fish for scallops in species identification. | SN/T 3328-2012: Protocol of identification of scallops. Simple-sequence repeats (SSR)---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of identification of scallops.Simple-sequence repeats (SSR)
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Scallop species identification methods SSR Act
Protocolofidentificationofscalops-Simple-sequencerepeats (SSR)
Issued on. 2012-12-12
2013-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Please note that some of the content of this document may involve patents. Distribution of this document
Structure does not bear the responsibility to identify these patents.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Yantai Exit Inspection and Quarantine, People's Republic of China Shandong Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Fangshao Qing, Yue Zhiqin, Yin Wei force, Liu Ning, Zhang Jingxuan, in red, Wang Ying, Xu Hongyan, bell, Lu Min, Duan Hui effect,
Luan Jing, Su Zhi Ping, Gengjin Pei.
Scallop species identification methods SSR Act
1 Scope
This standard specifies the scallop species SSR identification methods.
This standard applies to the entry and exit of chilled, dried, cooked, canned fish in species identification for scallop.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
SN/T 1193 genetic testing laboratory technical requirements
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Simple sequence repeat simplesequencerepeat; SSR
Microsatellite microsatelite
Flanking conservative to the core area of the nucleotide sequence into a 1-6 series end to end duplication. Microsatellite base in eukaryotes
Because the group is widely distributed, with rich polymorphism, codominant inheritance, the results are stable, etc., it has been in the population genetic analysis, parental identification, genetic
Biography linkage map, etc. are widely used.
3.2
Polymerase chain reaction polymernasechainreaction; PCR
In thermostable DNA polymerase and a pair of primers (tested with target nucleic acid molecule sequences homologous DNA fragments) at high temperature (DNA points
Sub-denatured) and low temperature (primers and the target nucleic acid molecule complex nature and thermostable DNA polymerase extension) alternating cycles of amplification of the target nucleic acid test
Child approach.
Principle 4
Preparation and screening of scallop, Chlamys nobilis, bay scallops and scallop small DNA fragments, using small insert library
Screening of microsatellite markers to construct small-scale genomic DNA library. Then use the sequence containing the oligonucleotide sequence specific repeating sequence
Primers were designed, the four scallops were specific PCR, the amplified product was subjected to agarose gel electrophoresis, amplification of a species
DNA fragment is determined scallop species. See Appendix A. Morphology scallops
5 Equipment and Reagents
5.1 Equipment
PCR, tissue grinder, clean table, ice maker, high-speed refrigerated centrifuge, water bath, small desktop centrifuge, a conventional refrigerator,
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