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SN/T 3170-2012: Detection and identification of Xylella fastidiosa
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| SN/T 3170-2012 | English | 419 |
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Detection and identification of Xylella fastidiosa
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SN/T 3170-2012
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Basic data | Standard ID | SN/T 3170-2012 (SN/T3170-2012) | | Description (Translated English) | Detection and identification of Xylella fastidiosa | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 16,129 | | Quoted Standard | SN/T 1860 | | Regulation (derived from) | National Quality Inspection (2012) 237; industry standard filing Notice 2012 No. 9 (Total No. 153); | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the entry in the grape Pierce phytosanitary quarantine and identification of bacteria. This standard applies to entry plant seedlings, propagation material and plant products are grapes Pierce quarantine and identification of bacte | SN/T 3170-2012: Detection and identification of Xylella fastidiosa---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection and identification of Xylella fastidiosa
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Pierce grape quarantine and identification of bacteria
Issued on. 2012-05-07
2012-11-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Beijing Entry-Exit Inspection and Quarantine Bureau, People's Republic of China Tianjin Entry-Exit Inspection and
Quarantine Bureau.
The main drafters of this standard. Deng Cong Liang, Zhang Kai, Gao Na, Lv Yufeng Liang Miao, Liu Peng.
Pierce grape quarantine and identification of bacteria
1 Scope
This standard specifies the entry in the phytosanitary quarantine and identification of grape Pierce bacteria.
This standard applies to entry quarantine and identification of plant seedlings, plant propagation material and product grape Pierce bacteria.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
SN/T 1860 plant virus immune electron microscopy method
Principle 3
3.1 grape Pierce bacteria taxonomic status
Grape Pierce germs scientific name Xylellafastidiosa Welsetal, 1987, the genus Prokaryotae (Procaryotae), thin-walled
Bacteria door (PhylumGracilicutes), xylem small genus (Xylella).
3.2 based quarantine and identification
In bacteria characteristic symptoms on the host, pathogen morphological characteristics and cultural characteristics, as well as the characteristics of the bacteria species genome sequence as Portugal
Pierce grapes quarantine pathogen identification based, if necessary, assisted Pathogenicity Test (see Appendix A).
4 equipment, reagents and utensils
4.1 apparatus, equipment, utensils
Clean benches, biological incubator, electronic scales, microplate reader, PCR instrument, gel imager, water bath, high-speed refrigerated centrifuge, high pressure
Autoclave, transmission electron microscopy, refrigerators, liquid nitrogen tanks, scalpel blades, white disk humidors, mortar, micropipette (adjustable range.
2.5μL ~ 1000μL), tip (10μL, 100μL, 1000μL), centrifuge tubes (1.5mL), PCR tubes, ELISA plate.
4.2 Reagents and culture medium
Test required medicines were of analytical grade.
Test reagent used in succinic acid - citrate - phosphate buffer, and its method of preparation see Appendix B.
PD2 used in the test medium, CS20 medium, PW medium, SPW medium, its method of preparation, see Appendix B.
5 grape Pierce bacteria identified
5.1 pairs antibody sandwich ELISA (DAS-ELISA) detection
The use of double-antibody sandwich enzyme-linked immunosorbent assay samples were screened (see Appendix C), if the test result is positive, the need for pathogens
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