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SN/T 2965-2011 English PDF

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SN/T 2965-2011: Criterion on detection of plant pathogenic fungi by molecular biology
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Basic data

Standard ID SN/T 2965-2011 (SN/T2965-2011)
Description (Translated English) Criterion on detection of plant pathogenic fungi by molecular biology
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 07.080
Word Count Estimation 13,173
Date of Issue 2011-05-31
Date of Implementation 2011-12-01
Quoted Standard GB/T 19495.2; GB/T 23632; GB/T 27403; SN/T 1127; SN/T 1155; SN/T 1193; SN/T 2080; SN/T 2126
Regulation (derived from) National-Quality-Inspection-accreditation (2011) 291
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the molecular biology of plant pathogenic fungi specification. This standard applies to plants and their products in the plant pathogenic fungi, separation and purification of biological molecules to detect plant pathogenic fungi.

SN/T 2965-2011: Criterion on detection of plant pathogenic fungi by molecular biology

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Criterion on detection of plant pathogenic fungi by molecular biology People's Republic of China Entry-Exit Inspection and Quarantine Standards Molecular biology of plant pathogenic fungi standardized testing Issued on. 2011-05-31 2011-12-01 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. People's Republic of China Zhuhai Entry-Exit Inspection and Quarantine Bureau, South China Agricultural University, Shenzhen Polytechnic. The main drafters of this standard. Peng Ren, who Daxing, Tsai Ping roots, Limin Hui, Jiang Zaide, Zhang Weidong, Marine Le, Chen Derong, Feng Yan. Molecular biology of plant pathogenic fungi standardized testing

1 Scope

This standard specifies the molecular biology of plant pathogenic fungi specification. This standard applies to plants and their products in the plant pathogenic fungi, separation and purification of plant pathogenic fungi molecular biology.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. GB/T 19495.2 technical requirements for GMO testing laboratories GB/T 23632 entry plant quarantine intercepted review procedures to identify harmful organism GB/T 27403 laboratory quality control of food molecular biology standardized testing SN/T 1127 Indian wheat bunt quarantine and identification of bacteria SN/T 1155 corn quarantine and identification of downy mildew SN/T 1193 genetic testing laboratory technical requirements SN/T 2080 sudden death of oak trees quarantine and identification of bacteria SN/T 2126 ryegrass bunt quarantine and identification of bacteria 3 Terms, definitions and abbreviations 3.1 Terms and Definitions The following terms and definitions apply to this document. 3.1.1 Sample sample One or more parts taken from a whole, is intended to provide the whole information, usually as the basis for the judgment as a whole. 3.1.2 Polymerase chain reaction polymerasechainreaction; PCR A molecular biological experiments in vitro enzymatic synthesis of specific DNA fragments, mainly low-temperature annealing and extension of suitable temperature from a high temperature denaturation Three steps repeated thermal cycling constitution. the template DNA is first denatured by high temperature single-stranded, DNA polymerase and at a suitable temperature, two Primers and annealing period of two complementary sequences on the template DNA strand occurs, followed by catalytic DNA polymerase in four deoxyribose Nucleotide triphosphate (dNTPs) as substrate, annealing of primers to be extended. And so forth, so that is located in two DNA fragments of known sequences It was amplified geometrically. 3.1.3 Nested polymerase chain reaction nestedPCR; nPCR Nested PCR The use of two sets of PCR primers (nested primers) two rounds of PCR amplification of a molecular biology experimental techniques response. In this technology Surgery, first with a pair of external primers for PCR amplification of the first round, and then using the first pair of primers to amplify the DNA sequence inside a pair Inner primers amplified again, so called nested PCR. By using two pairs of primers and conducted two rounds of amplification reactions, sensitization test

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