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SN/T 2497.5-2010: Test method of import and export dangerous chemicals. Part 5: Unscheduled DNA synthesis (UDS) test with testicle cells
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| SN/T 2497.5-2010 | English | 159 |
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Test method of import and export dangerous chemicals. Part 5: Unscheduled DNA synthesis (UDS) test with testicle cells
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SN/T 2497.5-2010
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Basic data | Standard ID | SN/T 2497.5-2010 (SN/T2497.5-2010) | | Description (Translated English) | Test method of import and export dangerous chemicals. Part 5: Unscheduled DNA synthesis (UDS) test with testicle cells | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | G09 | | Classification of International Standard | 13.300 | | Word Count Estimation | 6,653 | | Date of Issue | 2010-03-02 | | Date of Implementation | 2010-09-16 | | Adopted Standard | OECD NO.486-1997, MOD | | Regulation (derived from) | National Quality Inspection (2010) 98; industry standard filing Notice 2010 No. 5 (No. 125 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the import and export of dangerous chemicals testicular cells UDS test terminology and definitions, test theory, test procedures and test results. This standard applies to import and export of dangerous chemicals testicular cells induced animal testing DNA damage and repair. | SN/T 2497.5-2010: Test method of import and export dangerous chemicals. Part 5: Unscheduled DNA synthesis (UDS) test with testicle cells
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Test method of import and export dangerous chemicals.Part 5. Unscheduled DNA synthesis (UDS) test with testicle cells
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of dangerous chemicals - Test methods
Part 5. testicular cells UDS test
Issued on. 2010-03-02
2010-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 2497 "Test Methods for Chemical Safety & Exports dangerous" series of standards is divided into 29 parts.
--- Part 1. mammalian liver cells in vivo DNA synthesis (UDS) test;
--- Part 2. plaque-forming cells (PFC) test;
--- Part 3. Large Zhuan reproduction test;
--- Part 4. Saccharomyces cerevisiae mitotic recombination test;
--- Part 5. testicular cells UDS test;
--- Part 6. mammalian cells in vitro sister chromatid exchange test;
--- Part 7. mouse ear swelling test;
Part --- Article 8. fossa lymph node assay;
--- Part 9. Determination of serum hemolysin test;
Part --- Article 10. T lymphocyte proliferation assay test;
--- Part 11. germline mutation assay;
--- Part 12. single cell gel electrophoresis test;
--- Part 13. fluorescence in situ hybridization;
Part --- Article 14. SDS- polyacrylamide gel electrophoresis;
--- Part 15. PCR-SSCP experiment;
Part --- Article 16. Western-Blot test;
--- Part 17. Behavior of mammalian toxicology tests;
--- Part 18. Detection of DNA adducts;
--- Part 19. NorthernBlot experiment;
--- Part 20. Bradford protein assay;
--- Part 21. agarose gel electrophoresis;
--- Part 22. Tm value determination method of DNA;
--- Part 23. organelle separation experimental methods;
--- Part 24. cellular immunity in vitro assay method;
--- Part 25. humoral immune function tests;
--- Part 26. macrophage function tests;
--- Part 27. Flow cytometry of apoptosis;
--- Part 28. Application shuttle plasmid mutations Inspection;
--- Part 29. Biochemical Oxygen Demand (BOD) was measured.
This section SN/T 2497 Part 5.
The partial modification of the use of the Organization for Economic Cooperation and Development (OECD) Chemicals testing guidelines NO. 486 (1997) "in vivo mammalian hepatocytes
Outside the cellular DNA synthesis (UDS) test ", the technical content and the method described above exactly the same, in a standard text format press
GB/T 1.1-2000 made editorial changes.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section is responsible for drafting unit. People's Republic of China Tianjin Entry-Exit-Entry Inspection and Hunan People's Republic of China
Quarantine Bureau.
The main drafters of this section. Li Xue Yang, Wangli Bing, Chenqing Jun, in Yanjun, Zhao Zhuo, Zhou Lei.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Import and export of dangerous chemicals - Test methods
Part 5. testicular cells UDS test
1 Scope
SN/T 2497 This section specifies the terms and definitions testicular cells UDS test the import and export of dangerous chemicals, the principle of the test, the test
Test procedures and test results.
This section applies to the import and export of dangerous chemicals to induce animal testicular cells detect DNA damage and repair.
2 Terms and definitions
The following terms and definitions apply to this section.
2.1
Silver Net nuclear grain (NNG) higher than the cells of the laboratory historical negative control values.
2.2
Active quantitative determination, calculation of radiation from the developing UDS UDS test cell nuclear silver grains (NC) is equal to minus nuclear area
The average number of silver particles within the cytoplasm (CG), namely NNG. NC-CG. NNG calculated by the number of cells, and then a parallel culture or cultures
The cell NNG summary.
2.3
After the removal of DNA containing caused by chemical substances or physical damage to a section of DNA, carried out repair synthesis.
Principle 3
Testicular cells UDS test using somatic cells in vivo mammalian directs biological genetic toxicology, genetics primary end point
DNA damage. Since the test for detection of the whole genome, high sensitivity of this test provides mutagenic activity of the lack of specific information
It may be compensated.
UDS test showed that mammalian testicular cells, DNA DNA damage zone by the chain after removal by chemical or physical factors induce
Repair synthesis. This test is usually detected by autoradiography with 3H-TdR (thymidine) incorporation of testicular cell DNA. With a liquid scintillation counter
Comparison number, radioimmunoassay interference from developing method of S-phase cells insensitive.
Test Method 4
4.1 Preparation
4.1.1 animal selection
We should just use common strains of healthy adult animals. Preferred rats, can also choose other appropriate mammals. In the beginning of the trial,
Animal weight difference should not exceed ± 20% of the average weight of each sex.
4.1.2 Feeding Conditions
Animal room temperature should be 22 ℃ ± 3 ℃, relative humidity is generally maintained at 30% to 70%, except in a clean animal room, the relative humidity
Good control in 50% to 60%. Light artificial lighting, 12h alternating light and dark control. Fed with normal laboratory diet, limited access to water. Such as
If the test was incorporated into the feed were exposed, you should select the appropriate feed in order to ensure adequate mixing with the test substance. Animals may be kept separate or the same sex
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