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SN/T 2482-2010: Identification of potato witches broom phytoplasma
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| SN/T 2482-2010 | English | 239 |
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Identification of potato witches broom phytoplasma
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SN/T 2482-2010
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Basic data | Standard ID | SN/T 2482-2010 (SN/T2482-2010) | | Description (Translated English) | Identification of potato witches broom phytoplasma | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 07.080 | | Word Count Estimation | 9,982 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Regulation (derived from) | National Quality Inspection [2010] No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the potato arbuscular quarantine phytoplasma identification methods. This standard applies to all entry Branch potato, potato products, such as tissue culture and virus-free seedling potato germplasm blackbody arbuscular plant quarantine and identification. | SN/T 2482-2010: Identification of potato witches broom phytoplasma---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Identification of potato witches "broom phytoplasma
Exit inspection and quarantine industry standard book People's Republic of China
Potato arbuscular quarantine and identification of phytoplasma
Issued on. 2010-01-10
2010-07-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
The Standard Appendix A and Appendix B are informative.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard by the People's Republic of China Liaoning Entry Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine is responsible for drafting.
The main drafters of this standard. Wang Xiufen, Zhao Wenjun, Liu Wei, Jin Peng fire, Mouhai Qing, Zhou Ming, Zhu Shuifang.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Potato arbuscular quarantine and identification of phytoplasma
1 Scope
This standard specifies the method for inspection and identification of potato arbuscular phytoplasma.
This standard applies to all entry potato, potato products, potato plantlets and seedlings and other virus-free potato germplasm arbuscular phytoplasma quarantine
Identification.
Principle 2
2.1 scientific name and taxonomic status
Name. Potatowitches'broomphytoplasma
Abbreviation. PWB
Taxonomic position. Bacteria bound (Bacteria), Firmicutes (Firmicutes), flexible membrane Proteobacteria (Molicutes), non-steroidal Mycoplasma Head
(Cloverproliferationgroup, 16SrⅥ).
Additional information see Appendix A.
2.2 Identification of principle
Molecular biological characteristics of potato arbuscular phytoplasma based primarily on standard methods for identification.
3 apparatus and appliances
3.1 Equipment. PCR instrument, clean benches, electrophoresis, gel imaging analyzer, refrigerated centrifuge, electronic scales (1/10000g),
pH meter, water bath pot, oscillators.
3.2 appliances; adjustable pipette (0μL ~ 20μL, 20μL ~ 200μL, 200μL ~ 1000μL) and related tips, mortar tubes
(1.5mL, 10mL), PCR tubes (0.2mL), graduated cylinders, beakers tweezers.
4 Reagents
4.1 slurry
Disodium hydrogen phosphate (Na2HPO4) 0.1mol/L
Sodium dihydrogen phosphate (NaH2PO4) 0.03mol/L
EDTA 10mmol/L (pH8.0)
Sucrose 10% (mass concentration)
PVP-40 2% (mass concentration)
Adjusted to pH 7.6, followed by filtered sterilization.
Before using 0.15% (mass concentration) bovine serum albumin (BSA) and 1mmol/L ascorbic acid, vitamin C.
4.2 CTAB buffer
CTAB 2% (mass concentration)
Tris-HCl 100mmol/L (pH8.0)
EDTA 20mmol/L (pH8.0)
Sodium chloride (NaCl) 1.4mol/L
PVP-40 1% (mass concentration)
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