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| SN/T 1999-2007 | English | 279 |
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Protocol of antigen captured enzyme-linked immunosorbent assay for bovine viral diarrhoea-muscosal disease
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SN/T 1999-2007
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PDF similar to SN/T 1999-2007
Basic data | Standard ID | SN/T 1999-2007 (SN/T1999-2007) | | Description (Translated English) | Protocol of antigen captured enzyme-linked immunosorbent assay for bovine viral diarrhoea-muscosal disease | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 7,772 | | Date of Issue | 2007-12-24 | | Date of Implementation | 2008-07-01 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | ?Industry Standard Announcement 2008 No.2 (Total No.98) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the bovine viral diarrhea mucosal disease antigen capture enzyme-linked immunosorbent assay (ELISA) method of operation. This standard applies to bovine viral diarrhea mucosal disease antigen detection. | SN/T 1999-2007: Protocol of antigen captured enzyme-linked immunosorbent assay for bovine viral diarrhoea-muscosal disease
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Protocol of antigen captured enzyme-linked immunosorbent assay for bovine viral diarrhoea-muscosal disease
Exit inspection and quarantine industry standard book People's Republic of China
Bovine viral diarrhea - mucosal disease antigen capture enzyme-linked immunosorbent
Assay procedures
Posted 2007-12-24
2008-07-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine.
The main drafters of this standard. Dong Zhizhen, Hou Yanmei, Zhao Xiangping, Xiao Yan, Huo Lei.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Bovine viral diarrhea - mucosal disease antigen capture enzyme-linked immunosorbent
Assay procedures
1 Scope
This standard specifies the bovine viral diarrhea - mucosal disease antigen capture enzyme-linked immunosorbent assay (ELISA) method of operation.
This standard applies to bovine viral diarrhea - mucosal disease antigen detection.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (neq ISO 3696)
Principle 3
To border disease virus-specific antibody-coated microtiter plates, while adding BVDV detection of monoclonal antibodies and the sample, the sample that is with two anti
Binding, antigen-antibody conjugate is formed, the plate was washed and then secondary antibody (binding to an antigen-antibody complex) and the substrate, by measuring the optical density
The determination results of samples.
4 test material
4.1 bovine viral diarrhea - mucosal disease antigen capture ELISA diagnostic kit
2 ℃ ~ 8 ℃ preservation. Kit should include the following items.
a) bovine viral diarrhea - mucosal disease virus polyclonal antibody-coated plate;
b) detection of anti-BVDV monoclonal antibodies;
c) BVDV positive control;
d) BVDV negative control;
e) Horseradish peroxidase (HRP) labeled anti-mouse antibody cow;
f) 10 × sample diluent;
g) 10 × concentrated lotion;
h) TMB substrate solution;
i) Stop Solution.
4.2 sample handling
4.2.1 Organization
4.2.1.1 Use as fresh tissue samples, tissue can be stored for a month or long-term cryopreservation at 2 ℃ ~ 8 ℃. Most tissue samples
Good for the tonsils, spleen, small intestine and lung.
4.2.1.2 using scissors to cut up the sample 1g ~ 2g (2mm ~ 5mm).
4.2.1.3 The sample was cut into 10mL centrifuge tube was added 5mL sample diluent (1 ×), mixing at room temperature for sense 1h ~ 2h.
4.2.1.4 1500r/min centrifugal 10min, supernatant as detected by draw 50μL sample. Chapter 7 test.
4.2.2 peripheral blood leukocytes
4.2.2.1 heparin or EDTA anticoagulated blood sample 10mL, 2000r/min centrifugal 15min ~ 20min.
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