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| SN/T 1970-2007 | English | 839 |
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Determination of dexamethasone, betamethasone, triameinolone and flumethasone residues in foodstuffs of animal origin for import and export. Enyme-linked immunosorbent assay
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SN/T 1970-2007
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PDF similar to SN/T 1970-2007
Basic data | Standard ID | SN/T 1970-2007 (SN/T1970-2007) | | Description (Translated English) | Determination of dexamethasone, betamethasone, triameinolone and flumethasone residues in foodstuffs of animal origin for import and export. Enyme-linked immunosorbent assay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050 | | Word Count Estimation | 21,286 | | Date of Issue | 2007-08-06 | | Date of Implementation | 2008-03-01 | | Quoted Standard | GB/T 6379; GB/T 6682 | | Regulation (derived from) | National-accreditation-Science [2011] 63 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the foods of animal origin, dexamethasone, betamethasone, prednisolone and fluorine hydroxyl Flumethasone residual enzyme-linked immunoassay. This standard applies to meat tissue, aquatic products, milk and milk powder dexamethasone, betamethasone, prednisolone and fluorine hydroxyl Flumethasone residues. | SN/T 1970-2007: Determination of dexamethasone, betamethasone, triameinolone and flumethasone residues in foodstuffs of animal origin for import and export. Enyme-linked immunosorbent assay
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Determination of dexamethasone, betamethasone, triameinolone and flumethasone residues in foodstuffs of animal origin for import and export.Enyme-linked immunosorbent assay
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of animal-derived foods dexamethasone,
Betamethasone, triamcinolone and double fluorine dexamethasone
Residue Determination ELISA
Posted 2007-08-06
2008-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard and Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Zhejiang Exit Inspection and Quarantine.
The main drafters. Zhang Xiaofeng, Shi Weiliang, Zhu Zhenjiang, Wu Shan, He Yongqiang, Dong Qiang.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export of animal-derived foods dexamethasone,
Betamethasone, triamcinolone and double fluorine dexamethasone
Residue Determination ELISA
1 Scope
This standard specifies the foods of animal origin, dexamethasone, betamethasone, enzyme-linked immunoassay fluorine hydroxyl prednisolone and dexamethasone double Residues
Setting Method.
This standard applies to meat tissue, fish, milk and milk powder dexamethasone, betamethasone, triamcinolone and double fluorine dexamethasone residues
Determination of the amount.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequently
Some amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Study whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Precision GB/T 6379 of test methods to determine the repeatability and reproducibility of a standard test method by inter-laboratory
Laboratory use specifications and test methods GB/T 6682 Analysis
3 Sample preparation and preservation
3.1 Sample Preparation
Tissue samples. all the samples taken from a representative sample, mix, separated by quartering reduction of not less than 1000g sample, removing grease
Fat, full minced, mixed, packaging, sealing, and be identified.
Milk and milk powder. take the original packaging from a representative sample, mix thoroughly by quartering shrink separated not less than 500g sample loading
Clean airtight container, then sealed and marked with identification.
3.2 Sample preservation
The samples were stored at -20 ℃ ~ -18 ℃ condition.
4 Method summary
In acetonitrile, the three-phase liquid-liquid extraction system meta-phosphoric acid, n-hexane and dichloromethane to extract the residual tissue dexamethasone, betamethasone
And fluorine triamcinolone and the like. After processing the samples residual hormones and enzyme-labeled dexamethasone dexamethasone jointly compete for binding antibodies, simultaneously
Antibodies bind to dexamethasone coated with sheep anti-rabbit IgG antibody microplate, by washing to remove unbound enzyme-labeled residues and ground
Dexamethasone was added chromogenic substrate, absorbance was measured absorbance is inversely proportional to the analyte concentration using a microplate reader, the absorbance is worth a try
The content of the sample residue.
5 Materials and Reagents
Unless otherwise indicated, all reagents were of analytical grade water as a water GB/T 6682 regulations.
Dexamethasone 5.1 detection kit (see Appendix A).
5.2 metaphosphate.
5.3 acetonitrile.
5.4 n-hexane.
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