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 SN/T 1960-2007: Screening method of sulfonamide residues in animal original food for import and export. Enzyme linked immunosorbent assay method
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 Basic data             | Standard ID | SN/T 1960-2007 (SN/T1960-2007) |           | Description (Translated English) | Screening method of sulfonamide residues in animal original food for import and export. Enzyme linked immunosorbent assay method |           | Sector / Industry | Commodity Inspection Standard (Recommended) |           | Classification of Chinese Standard | X04 |           | Classification of International Standard | 67.050 |           | Word Count Estimation | 18,115 |           | Date of Issue | 2007-08-06 |           | Date of Implementation | 2008-03-01 |           | Quoted Standard | GB/T 6682 |           | Regulation (derived from) | Accredidation-Technology-Letter [2015] No. 247 |           | Issuing agency(ies) | General Administration of Customs |           | Summary | This standard specifies the seven kinds of food of animal origin sulfonamides residues ELISA method. This standard applies to pork, chicken, liver, eggs, fish, milk, seven kinds of sulfonamides residues screening tests. | SN/T 1960-2007: Screening method of sulfonamide residues in animal original food for import and export. Enzyme linked immunosorbent assay method---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
 Screening method of sulfonamide residues in animal original food for import and export.Enzyme linked immunosorbent assay method
Exit inspection and quarantine industry standard book People's Republic of China
Import and export of animal-derived foods sulfa drug residue
Detection method allowance of ELISA
Posted 2007-08-06
2008-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
 ForewordThe Standard Appendix A, Appendix B, Appendix C and Appendix D are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. Tianjin People's Republic of China Exit Inspection and Quarantine, Tianjin University of Science and Technology, the People's Republic of China Hubei
Entry Inspection and Quarantine Bureau.
The main drafters of this Standard. Kit Cheng, Wang Shuo, Zhang Hongwei, Hu Zhong, Wu Yanhui, Yao Xia, Zhang Hongyan, Zhang Yan, Zhang Jun.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Import and export of animal-derived foods sulfa drug residue
Detection method allowance of ELISA1 ScopeThis standard specifies the ELISA method in food of animal origin of seven sulfonamides residues.
This standard applies to Seven sulfa drugs residues in pork, chicken, liver, eggs, fish, milk screening test.2 Normative referencesThe following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
Laboratory use specifications and test methods GB/T 6682 analysis.3 Determination3.1 Method summary
The samples were extracted with sulfa drugs extracts were detected using a competitive enzyme-linked immunosorbent assay.
3.2 Reagents and materials
Unless otherwise requested, all reagents were of analytical grade water as a water GB/T 6682 regulations.
3.2.1 sulfa drugs ELISA assay kit 1) (see Appendix A).
1) sulfa drugs ELISA kit provided by the Tianjin Biochip Technology Co., Ltd. under the trade name product. This gives
Information is for the convenience of users of this standard does not imply endorsement of the product. If other equivalent product has the same effect, you can use this
Some equivalent.
3.2.2 acetone.
3.2.3 ethanol.
3.2.4 disodium hydrogen phosphate (Na2HPO4 · 12H2O).
3.2.5 sodium dihydrogen phosphate (NaH2PO4 · 2H2O).
3.2.6 sodium chloride.
3.2.7 masking agent dilution. 0.5% bovine serum albumin, 1% bovine serum albumin, when used in the preparation.
3.2.8 Standard. sulfamethazine different mouth oxazole, Sulfathiazole, sulfamethoxydiazine, sulfa methoxy-triazine, sulfasalazine, sulfamethizole, sulfonamides
Chlorine of triazine (English names are. sulfisozole, sulfathiazole, sufameter, sulfamethoxypyridazine, sulfapyridine,
sulfamethizole, sulfachlorpyridazine; CAS Code. 127-69-5,72-14-0,651-06-9,80-35-3,144-83-2,
144-82-1,80-32-0). not less than 99% purity.
3.2.9 Standard stock solution. formulated as 10mg/L stock solution of methanol, -20 ℃ preservation, valid for three months.
Cryopreservation agents, stand at room temperature should not exceed 4h, when used, should each solution to reach room temperature.
3.3 instruments and equipment
3.3.1 grinder.
3.3.2 homogenizer.
3.3.3 scales (accurate to 0.001g).
3.3.4 vortex mixer.
 
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