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Detection of lactic acid bacteria in food for import and export. Part 2: : PetrifilmTM enumeration method
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SN/T 1941.2-2007
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Basic data | Standard ID | SN/T 1941.2-2007 (SN/T1941.2-2007) | | Description (Translated English) | Detection of lactic acid bacteria in food for import and export. Part 2: : PetrifilmTM enumeration method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X04 | | Classification of International Standard | 67.050; 07.100.30 | | Word Count Estimation | 8,823 | | Date of Issue | 2007-08-06 | | Date of Implementation | 2008-03-01 | | Regulation (derived from) | Accredidation-Technology-Letter [2015] No. 247 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the Lactobacillus Petrifilm method. This standard applies to natural or adding lactic acid bacteria in food and raw materials Determination of lactic acid bacteria. | SN/T 1941.2-2007: Detection of lactic acid bacteria in food for import and export. Part 2: : PetrifilmTM enumeration method
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Detection of lactic acid bacteria in food for import and export.Part 2 .. PetrifilmTM enumeration method
Exit inspection and quarantine industry standard book People's Republic of China
Lactic acid bacteria in food import and export inspection methods
Posted 2007-08-06
2008-03-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1941 "lactic acid bacteria in food import and export inspection method" is divided into three parts.
--- Part 1. Separation and counting method;
--- Part 2. PetrifilmTM test plate method;
--- Part 3. Lactobacillus PCR method.
This section SN/T 1941 Part 2.
This section of Appendix A normative appendix.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
This section was drafted by. People's Republic of China Liaoning Entry-Exit-Entry Inspection and Heilongjiang People's Republic of China
Quarantine Bureau.
The main drafters of this section. Wu Bin, Qin Cheng, Wang Mei, Li Sulong, Wang Gang, Qi Zhenyu.
The first part of the Department of Inspection and Quarantine issued by industry standards.
Lactic acid bacteria in food import and export inspection methods
1 Scope
This section SN/T 1941 specifies the PetrifilmTM Determination of lactic acid bacteria.
This section applies to natural or added food and feed Determination of lactic acid bacteria of lactic acid bacteria.
2 Terms and definitions
The following terms and definitions apply to SN/T 1941 to the present section.
2.1
Substrate A defined previously prepared medium system containing a standard cold water soluble gelling agent and triphenyl tetrazolium chloride
(TTC) indicator, can enhance the effect of colony counts. After MRS broth after sample preparation is good, in microaerophilic culture conditions can be acid
Bacteria counts.
3 equipment and materials
3.1 Incubator. 36 ℃ ± 1 ℃ and 25 ℃ ± 1 ℃.
3.2 micropipette 1000μL.
3.3 microaerobic culture apparatus. optimal microaerobic conditions of 5% oxygen, 10% carbon dioxide and 85% nitrogen. Duplex manometer can appliance
Microaerophilic incubator, anaerobic tank, candle jar, bag or other alternative means.
3.4 Balance. Range 2kg, a sense of the amount of 0.1g.
3.5 Sterilization of sample preparation equipment. straw, sampling spoon, scissors, can opener.
3.6 Sample dilution bottle. 500mL sample dilution bottles.
3.7 platen.
4 media and reagents
Unless otherwise specified, reagents were analytical or biochemical reagents, water is distilled water.
4.1 phosphate buffer (see Section A.1 chapter).
4.2 MRS broth (see Section A.2 chapter).
4.3 PetrifilmTM total bacteria test piece.
5 test methods
5.1 Method summary
Renewable hydrate a dry film PetrifilmTM for lactic acid bacteria count, which consists of a film composed of two layers, the lower layer of polyethylene
Printed on the film and covered with a mesh required for the growth of lactic acid bacteria culture medium, the upper is a polypropylene film. Just to be inoculated using 1mL
Dilution of the sample being measured on the lower medium, cover the top of the polypropylene film, a polyethylene layer on the media at this time due to raw water
A hydrate, for the growth of lactic acid bacteria. PetrifilmTM test piece is the use of TTC (triphenyltetrazolium chloride) as an indicator of colonies, can
Lactic acid present in food counts.
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