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SN/T 1908-2007: Protovol of isolation and identification for parasite eggs or oocysts from kimchi and other plant foods
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1908-2007 | English | 289 |
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Protovol of isolation and identification for parasite eggs or oocysts from kimchi and other plant foods
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SN/T 1908-2007
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Basic data | Standard ID | SN/T 1908-2007 (SN/T1908-2007) | | Description (Translated English) | Protovol of isolation and identification for parasite eggs or oocysts from kimchi and other plant foods | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | C53 | | Classification of International Standard | 07.100.30 | | Word Count Estimation | 9,935 | | Date of Issue | 2007-05-23 | | Date of Implementation | 2007-12-01 | | Regulation (derived from) | National-accreditation-Science [2011] 63 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the kimchi and other plant -derived foods parasite eggs (including roundworm Ascaris, Hookworms Ancylostoma, hair filaria Trichuris, eggs and wool nematodes Trichostrongylus Isopsopoa Isospora oocysts, etc.) separation, morphological Ascaris eggs fluorescent PCR identification and authentication methods. This standard applies to pickles, chili sauce, barbecue sauce and other foods of plant origin parasite eggs inspection sample preparation, separation, using a microscope for morphological identification, as well as for PCR detection of Ascaris accurate identification. | SN/T 1908-2007: Protovol of isolation and identification for parasite eggs or oocysts from kimchi and other plant foods
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protovol of isolation and identification for parasite eggs or oocysts from kimchi and other plant foods
Exit inspection and quarantine industry standard book People's Republic of China
Pickles and other plant-derived foods parasite eggs
Isolation and identification procedures
Posted 2007-05-23
2007-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
Appendix A of this standard is a normative appendix, Appendix B is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. China Inspection and Quarantine Science Research Institute, People's Republic of China Shandong Exit Inspection and Quarantine, the People's Republic of China
Exit Inspection and Quarantine and the State of Liaoning.
The main drafters of this standard. Wushao Jiang, Lin Xiangmei, Labyrinth, GLACIOLOGY, Han Xueqing, Mei Lin, Liu, Jia Guang Yue, Cao Juan occasion, Li Fengqi.
This standard is the first release of the entry-exit inspection and quarantine industry standards.
Pickles and other plant-derived foods parasite eggs
Isolation and identification procedures
1 Scope
Ascaris eggs fluorescent PCR identification methods.
This standard applies to the sample preparation pickles, chili sauce, barbecue sauce and other plant-derived foods when parasite eggs inspection, separation, use of microscope
Morphological identification, as well as the detected fluorescence PCR Ascaris accurate identification.
2 Terms and definitions
The following terms and definitions apply to this standard.
2.1
From a single sample point a small sample was drawn.
2.2
Parts from the same batch of samples obtained through mixing a number of samples.
2.3
Consists of all or part of the original sample was further homogenized samples for analysis or testing.
2.4
Dispensation from the original sample for future reference of a certain number of samples.
2.5
Fluorescent PCR reaction, the fluorescent signal reaches the threshold set number of cycles experienced.
3 Reagents
Unless otherwise specified, the use of biological and chemical reagents were of analytical grade.
Saturated sodium chloride solution 3.1
Take sodium chloride (specific gravity 1.18 ~ 1.20) 400g, added to 1000mL water, heated to boiling sodium chloride is completely dissolved.
3.2 0.5% SDS solution
Weigh SDS (sodium dodecyl sulfate) 5.0g, was added 1000mL of water dissolved.
3.3 Identification of fluorescent PCR reagent
3.3.1 Ascaris eggs PCR assay primers, probes, using software BeaconDesigner design and BLAST analysis, verification
Roundworm parasite superfamily of universal primers and probes.
3.3.2 commercialization RealtimePCR reaction mix.
3.3.3 positive control samples. The Ascaris female, anatomy laboratory, part of the acquisition of the uterus using the kit to extract genomic DNA,
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