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SN/T 1816-2013 English PDF

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SN/T 1816-2013: Detection of genetically modified components. Tomato test methods
Status: Valid

SN/T 1816: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 1816-2013English319 Add to Cart 3 days [Need to translate] Detection of genetically modified components. Tomato test methods Valid SN/T 1816-2013
SN/T 1816-2006English439 Add to Cart 4 days [Need to translate] Tomato genetically modified qualitative PCR detection method Obsolete SN/T 1816-2006

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Basic data

Standard ID SN/T 1816-2013 (SN/T1816-2013)
Description (Translated English) Detection of genetically modified components. Tomato test methods
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B31
Classification of International Standard 67.080.20
Word Count Estimation 12,197
Older Standard (superseded by this standard) SN/T 1816-2006
Quoted Standard GB/T 6682; GB/T 19495.1; GB/T 19495.2; GB/T 19495.3; SN/T 1194
Regulation (derived from) AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall)
Issuing agency(ies) General Administration of Customs
Summary This standard specifies the genetically modified tomato and its products in conventional PCR and real-time PCR detection method. This standard applies to tomatoes and turn the qualitative detection of gene genetically modified products.

SN/T 1816-2013: Detection of genetically modified components. Tomato test methods

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Detection of genetically modified components.Tomato test methods People's Republic of China Entry-Exit Inspection and Quarantine Standards Instead of the SN/T 1816-2006 Detection methods to detect genetically modified tomato Issued on. 2013-03-01 2013-09-16 implementation People's Republic of China The State Administration of Quality Supervision, Inspection and Quarantine released

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules. Instead of the standard SN/T 1816-2006 "Genetically Modified Tomato Qualitative PCR detection method." This standard compared with SN/T 1816-2006, the main technical changes are as follows. --- Standardized terminology and way of expression; --- Revised standard original structure; --- Terminology and abbreviations reference "GMO testing - General requirements and definitions" in the provisions of GB/T 19495.1; --- Increased by promoter FMV35S subject gene in a conventional PCR detection method; --- Increasing the real-time PCR detection methods and results to determine the method. Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. This standard was drafted. Xinjiang People's Republic of China Exit Inspection and Quarantine, Chinese Academy of Inspection and Quarantine. The main drafters. Zhang Xianglin, Wang, Chen Ying, Zhangjiang Guo, Zhang. This standard replaces the standards previously issued as follows. --- SN/T 1816-2006. Detection methods to detect genetically modified tomato

1 Scope

This standard specifies the tomato and its products are genetically modified conventional PCR and real-time PCR detection method. This standard applies to qualitative detection of genetically modified tomato products and genetically modified ingredients.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document. Laboratory use specifications and test methods GB/T 6682 Analysis GB/T 19495.1 GMO testing - General requirements and definitions GB/T 19495.2 technical requirements for GMO testing laboratories GB/T 19495.3 GMO detection of nucleic acid extraction and purification method SN/T 1194 to detect genetically modified plants and their products sampling and sample preparation methods 3 Terms, definitions and abbreviations Terminology GB/T 19495.1 defined, definitions and abbreviations apply to this document. 4 anti-pollution measures Measures to prevent cross-contamination in the testing process should be consistent with GB/T 19495.2 provisions. Principle 5 After extraction of the sample DNA, for transgenic tomato, exogenous gene gene sequence primers and probes by conventional PCR Or real-time fluorescence PCR, detected the presence of various internal and exogenous gene, to tomato and its products in the presence or absence of GMOs determination.

6 apparatus and appliances

6.1 Instrument Real-time PCR instrument, gene amplification device, electrophoresis, electrophoresis tank, clean benches, electronic scales (sense of volume 1mg), gel imager, Full warm bath, high-speed refrigerated centrifuges, autoclaves, ice maker, a vortex, water meter, low temperature refrigerators, nucleic acid protein analyzer, miniature Centrifuges, microwave. 6.2 Appliances And supporting micro-adjustable pipette tips (0.1μL ~ 2μL, 2.5μL ~ 10μL, 2μL ~ 20μL, 10μL ~ 100μL,

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