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| SN/T 1700-2006 | English | 239 |
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The animal fur Anthrax Ascoli reaction Procedures
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SN/T 1700-2006
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Basic data | Standard ID | SN/T 1700-2006 (SN/T1700-2006) | | Description (Translated English) | The animal fur Anthrax Ascoli reaction Procedures | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 6,613 | | Date of Issue | 2006-01-26 | | Date of Implementation | 2006-08-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry Standard Notice 2006 No. 4 (total of 76) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the animal hides, raw wool in Ascoli anthrax experiments (Ascoli test) detection methods. This standard applies to animal hides, wool anthrax antigen test. | SN/T 1700-2006: The animal fur Anthrax Ascoli reaction Procedures---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
The animal fur Anthrax Ascoli Reaction Procedures
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Released on.2006-01-26
Implementation of.2006-08-16
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Guangdong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Liang Gang, Lin Zhixiong, Yu Haiqiong, Chen Ru, Liu Linlin.
This standard is the first industry standard for entry-exit inspection and quarantine.
1 Scope
This standard specifies the Ascoli test test method for animal skin and anthrax in raw wool.
This standard applies to animal skin and original anthrax antigen test.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Terms and definitions
The following terms and definitions apply to this standard.
3.1
A precipitation assay for the detection of anthrax antigens in animal tissues using antiserum as a precipitin.
4 materials
4.1 Equipment
Autoclave, 50μL～200μL single channel pipette, ordinary refrigerator, immersion bottle, reaction tube, microscope, ultra clean table, constant temperature
Incubator, Gram stain solution.
4.2 skin (hair)-like infusion
See Appendix A for the preparation method.
4.3 diagnostic reagents
4.3.1 Anthrax Precipitin Standard Antigen. Bacillus anthracis powder antigen for positive and negative control trials, provided by the designated unit.
4.3.2 Anthrax precipitin positive serum. used for sample testing and positive control test, 4% sodium chloride should be added when detecting salted skin.
The unit is provided.
4.3.3 Anthrax negative serum. used for negative control trials, provided by the designated unit.
5 sample collection and processing
5.1 Sample Collection
The skin to be inspected and the original hair should be stored in a dedicated warehouse and designated place. Different batches of skin and raw wool should be stored strictly separately. Placed in each batch
In one place, each piece is numbered, and the original wool is individually packaged. Pick a piece of skin about 2g in the leg or underarm of each skin. The original hair is in each separate package.
A sample of 3 g was sampled, and if it was not individually packaged, 3 g of each of the 20 different points dispersed was sampled. Sample skin and sample hair should be filled with special sealing metal
Box or high temperature resistant waterproof sample bag, and numbered according to the original number.
5.2 Sample Processing
5.2.1 Sample disinfection
The test sample was placed in an autoclave along with the sample bag and sterilized at 103 kPa and 121 ° C for 30 min. Frozen skin, wet skin, fresh skin should be high
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