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Horse piroplasmosis micro complement fixation test operating procedures
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SN/T 1695-2006
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PDF similar to SN/T 1695-2006
Basic data | Standard ID | SN/T 1695-2006 (SN/T1695-2006) | | Description (Translated English) | Horse piroplasmosis micro complement fixation test operating procedures | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 10,122 | | Date of Issue | 2006-01-26 | | Date of Implementation | 2006-08-16 | | Quoted Standard | GB/T 6682 | | Regulation (derived from) | Industry Standard Notice 2006 No. 4 (total of 76) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the horse piroplasmosis micro complement fixation test method of operation. This standard applies to the horse equine piroplasmosis serological diagnosis. | SN/T 1695-2006: Horse piroplasmosis micro complement fixation test operating procedures---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Horse piroplasmosis micro complement fixation test operating procedures
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Majiaosis micro-complement binding test operation procedure
Released on.2006-01-26
Implementation of.2006-08-16
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard is mainly based on the OIE "Diagnostic Reagents and Vaccine Standards Manual" (2004) fifth edition, Chapter 2.5.6.
Appendix A, Appendix B and Appendix C of this standard are normative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Liaoning Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Jian Zhongyou, Fu Lei, Hu Chuanwei, Sun Yanfeng, Su Yongsheng, Sun Yingjie.
This standard is the first industry standard for entry-exit inspection and quarantine.
Majiaosis micro-complement binding test operation procedure
1 Scope
This standard specifies the method of operation for the micro-complement binding test of horse coccidiosis.
This standard applies to the serological diagnosis of equine animal horse coccidiosis.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
3 reagents and materials
3.1 Reagents
3.1.1 The distilled water used for the test is the tertiary water in GB/T 6682.
3.1.2 Diluent. Barbiturate buffer (see Chapter A.2).
3.1.3 Complement-bound antigen. provided by the designated unit, used according to the instructions.
3.14 Standard positive serum. provided by the designated unit, used according to the instructions.
3.1.5 Standard negative serum. provided by the designated unit, used according to the instructions.
3.1.6 Hemolysin. Freeze-dried product, dissolved in distilled water to the specified volume before use.
3.1.7 Complement. Freeze-dried product, dissolved in distilled water to the specified volume before use.
3.1.8 sheep red blood cell storage solution. choose healthy adult sheep, blood sampling in sterile jugular vein, 1.2 parts of aristoleine (see chapter A.1) plus 1 part of blood
Liquid, mix, store at 2 ° C ~ 8 ° C, use within 2 weeks.
3.2 Tested serum
3.2.1 Blood collection and separation of serum
Venous blood was collected according to a conventional method, and serum was aseptically separated. Serum must be fresh, free of protein flocs, no hemolysis, no spoilage.
3.2.2 Shipping and preservation of serum
Serum is shipped refrigerated. If it cannot be sent to the laboratory within 3 days, it should be shipped by freezing.
3.3 Materials
3.3.1 Glass test tube. 10mm × 75mm, round bottom clean reaction tube.
3.3.2 Test tube rack.
3.3.3 Micropipettes.
3.3.4 Adjustable continuous sampler.
3.3.5 96-well "U" type microplate.
4 method of operation
4.1 preliminary test
4.1.1 Preparation of 3% red blood cell suspension
4.1.1.1 After the sheep's whole blood is thoroughly mixed, take the required amount and wash it three times with the dilution solution, centrifuge at.2000r/min for 5min each time, and last time away.
Heart 10min.
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