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SN/T 1632.2-2013: Determinetion of Enterobacter sakazakii (Cronobacter spp.)from dehydrated powdered milk for export. Part 2: PCR method
Status: Valid SN/T 1632.2: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1632.2-2013 | English | 319 |
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Determinetion of Enterobacter sakazakii (Cronobacter spp.)from dehydrated powdered milk for export. Part 2: PCR method
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SN/T 1632.2-2013
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| SN/T 1632.2-2005 | English | 559 |
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Milk powder in Sakamoto Qi Enterobacter test methods - Part 2: PCR methods
| Obsolete |
SN/T 1632.2-2005
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PDF similar to SN/T 1632.2-2013
Basic data | Standard ID | SN/T 1632.2-2013 (SN/T1632.2-2013) | | Description (Translated English) | Determinetion of Enterobacter sakazakii (Cronobacter spp.)from dehydrated powdered milk for export. Part 2: PCR method | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X16 | | Classification of International Standard | 67.100 | | Word Count Estimation | 12,197 | | Older Standard (superseded by this standard) | SN/T 1632.2-2005 | | Quoted Standard | GB/T 6682; GB/T 27403-2008; SN/T 1632.1 | | Regulation (derived from) | National quality recognition (2013) 569 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the milk Enterobacter sakazakii (Craunot spp) of PCR detection method. This standard applies to milk Enterobacter sakazakii (Craunot spp.) In the rapid qualitative detection of other foods for implementation. | SN/T 1632.2-2013: Determinetion of Enterobacter sakazakii (Cronobacter spp.)from dehydrated powdered milk for export. Part 2: PCR method
---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
(. Cronobacter spp) Determinetion of Enterobacter sakazakii from dehydrated powdered milk for export.Part 2. PCR method
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1632.2-2005
Enterobacter sakazakii in milk powder exports (Craunot sp)
Test methods - Part 2. PCR methods
DeterminetionofEnterobactersakazaki (Cronobacterspp.) Fromdehydrated
powderedmilkforexport-Part 2. PCRmethod
Issued on. 2013-11-06
2014-06-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1632 "Enterobacter sakazakii in milk powder exports (Craunot sp) test methods" is divided into three parts.
--- Part 1. Separation and counting;
Part --- Article 2. PCR method;
--- Part 3. Fluorescent PCR method.
This section SN/T 1632 Part 2.
This section drafted in accordance with GB/T 1.1-2009 given rules.
The partial replacement of SN/T 1632.2-2005 "Enterobacter sakazakii in milk powder test methods - Part 2. PCR testing methods."
This section compared with the SN/T 1632.2-2005, the main technical changes are as follows.
--- The standard according to GB/T 1.1-2009 specification text format;
--- Modify the standard English name;
--- Deleted the definitions, terms and abbreviations;
--- Remove the separation medium crystal violet neutral red bile dextrose agar (VRBGA).
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
This part by the National Certification and Accreditation Administration of People's Republic of China and focal points.
This section was drafted by. People's Republic of China Tianjin Entry-Exit Inspection and Quarantine Bureau, Chinese Academy of Inspection and Quarantine.
The main drafters of this section. Zhang Xia, Liu Pei, Zhaogui Ming, Gao Qi Li, Wu winter snows, Zhang Haiying, Qu Peng, Zhao Liangjuan, Zhang Hongwei.
This part of the standard replaces the previous editions are.
--- SN/T 1632.2-2005.
Enterobacter sakazakii in milk powder exports (Craunot sp)
Test methods - Part 2. PCR methods
1 Scope
SN/T 1632 provisions of this part of the milk powder Enterobacter sakazakii (Craunot sp) of the PCR detection method.
This section applies to milk Enterobacter sakazakii (Craunot sp) the rapid qualitative detection of other foods may refer to.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB/T 27403-2008 food quality control laboratory of molecular biology standardized testing
SN/T 1632.1 milk Enterobacter sakazakii (Craunot sp) - Part 1. Separation and Counting
3 equipment and materials
3.1 Balance. Range 2kg, a sense of the amount of 0.1g.
3.2 water bath. to maintain the water temperature 44.5 ℃ ± 0.2 ℃.
3.3 Incubator. 35 ℃ ~ 37 ℃ and 43 ℃ ~ 45 ℃.
3.4 PCR instrument.
3.5 centrifuge.
3.6 UV gel imager.
3.7 electrophoresis.
3.8 pH meter.
3.9 Pipette. 2μL ~ 10μL, 10μL ~ 100μL, 20μL ~ 200μL, 100μL ~ 1000μL.
4 media and reagents
Test water should comply with GB/T 6682 stipulated a water.
4.1 Modified lauryl sulfate tryptone broth - vancomycin (mLST-Vm) (see Appendix A, A.1).
4.2 brain heart infusion (BHI) (see A.2).
4.3 nutrient broth (NB) (see A.3).
4.4 Primer SAKAF. 5'-GGGTTGTCTGCGAAAGCGAA-3 '; SAKAR. 5'GTCTTCGTGCTGCGAGTTTG-3'.
4.5 TaqDNA polymerase.
4.6 dNTP. dATP, dTTP, dCTP, dGTP.
4.7 10 × PCR buffer.
4.8 Enterobacter sakazakii control strains. ATCC29544, or equivalent strains.
4.9 DNA extraction kit. bacterial genome DNA extraction kit.
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