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Determination of tetrodotoxin in puffer fish for export. Part 2: Method of mouse bioassay
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SN/T 1569.2-2013
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Basic data | Standard ID | SN/T 1569.2-2013 (SN/T1569.2-2013) | | Description (Translated English) | Determination of tetrodotoxin in puffer fish for export. Part 2: Method of mouse bioassay | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | X20 | | Classification of International Standard | 67.120.30 | | Word Count Estimation | 12,174 | | Quoted Standard | GB/T 6682; GB 14925 | | Regulation (derived from) | AQSIQ notification issued in 2013 on the first batch of 179 entry-exit inspection and quarantine of industry standards; industry standard for filing Notice 2013 No. 9 (No. 165 overall) | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the export of household and similar electrical appliances (hereinafter referred to as household appliances) inspection procedures and requirements. This standard applies to the inspection and quarantine agencies and conformity asse | SN/T 1569.2-2013: Determination of tetrodotoxin in puffer fish for export. Part 2: Method of mouse bioassay
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Determination of tetrodotoxin in puffer fish for export.Part 2. Method of mouse bioassay
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Export puffer fish, tetrodotoxin detection method
Part 2. mouse bioassay
Part 2. Methodofmousebioassay
Issued on. 2013-03-01
2013-09-16 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
SN/T 1569 "export puffer fish, tetrodotoxin detection method" is divided into two parts.
--- The first part 1. ELISA method;
--- Part 2. mouse bioassay.
This section SN/T 1569 Part 2.
This section drafted in accordance with GB/T 1.1-2009 given rules.
This section proposed and managed by the National Certification and Accreditation Administration Committee.
Please note that some of the content of this document may involve patents. Release mechanism of the present document does not assume responsibility for the identification of these patents.
This section was drafted by. People's Republic of China Shandong CIQ.
The main drafters of this section. Wang Jing, Yang Lijun, Liu Heping, Li Zhaojie, Hu Qiaoru, Cong Weihong, when Wen Chun, Li Zhen, Liu Yumin.
Export puffer fish, tetrodotoxin detection method
Part 2. mouse bioassay
1 Scope
This section SN/T 1569 specifies the method for detecting biological export puffer fish, tetrodotoxin mice.
This section applies to export puffer fish muscle tissue, liver, skin detection of tetrodotoxin.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB 14925 Laboratory Animal Facilities and Environment
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1
Tetrodotoxin tetrodotoxin; TTX
A highly toxic poison dolphin fish and other organisms containing alkaloids, also known salamander toxins, toxins East, the formula
C11H17O8N3, relative molecular mass of 319, is an inhibitory nerve impulses by blocking the sodium channel non-protein neurotoxin.
3.2
Virulence toxicity
Pharmacy occur the nature and extent of the direct effect of a particular organism.
3.3
Rat units mouseunit; MU
For weighing 20g of specific pathogen free (SPF) Kunming male mice by intraperitoneal injection of 1mL tetrodotoxin extract it in
The minimum required amount of toxins death within 30min.
3.4
Intermediate lethal time mediandeathtime
The mice were dead by the time variable values in order of size, location in the middle of that value is the middle of the time of death.
4 Sample Storage
If you can not test the samples for inspection immediately, should be separately marked with a label source, date of sampling, variety and number, put a plastic bag sealed. After sampling
The following samples should be immediately placed in -18 ℃ cryopreservation.
5 measurement method
5.1 Method summary
According to the principle of tetrodotoxin soluble in acid solution, 2 times after sample preparation by boiling 0.5% acetic acid solution to extract, 20000g centrifugation
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