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SN/T 1543-2005 English PDF

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SN/T 1543-2005: GeneChip methods for identification of foodborne pathogens
Status: Obsolete
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PDF similar to SN/T 1543-2005


Standard similar to SN/T 1543-2005

YY/T 1529   YY/T 1528   YY/T 1533   SN/T 1538.2   SN/T 1538.1   

Basic data

Standard ID SN/T 1543-2005 (SN/T1543-2005)
Description (Translated English) GeneChip methods for identification of foodborne pathogens
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard C44
Classification of International Standard 07.100.01
Word Count Estimation 12,13
Date of Issue 2005-02-17
Date of Implementation 2005-07-01
Regulation (derived from) Industry standard filing Notice 2005 No. 4 (No. 64 overall)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the foodborne pathogens microarray detection methods. This standard applies to qualitative detection of foodborne pathogens and identification.

SN/T 1543-2005: GeneChip methods for identification of foodborne pathogens

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
GeneChip methods for identification of foodborne pathogens Book of the People's Republic of China Entry and Exit Inspection and Quarantine Foodborne pathogenic bacteria gene chip identification method Released on.2005-02-17 2005-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A and Appendix B of this standard are normative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shanghai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Liaoning Entry-Exit Inspection of the People's Republic of China Epidemic situation. The main drafters of this standard. Gu Ming, Cao Jijuan, Huang Xinhua, Han Wei. This standard is the first industry standard for entry-exit inspection and quarantine. Foodborne pathogenic bacteria gene chip identification method

1 Scope

This standard specifies the detection method of food-borne pathogenic gene chip. This standard applies to the qualitative detection and identification of foodborne pathogenic bacteria.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 1193 Genetic Testing Laboratory Technical Requirements 3 Terms, definitions and abbreviations 3.1 Terms and definitions The following terms and definitions apply to this standard. 3.1.1 A type of bacteria that is derived from food and agricultural products and can cause human health hazards. 3.1.2 Polymerase chain reaction, referred to as PCR. The principle is to use two short oligonucleotides as primers for the reaction, and the sequence of the two primers A specific site in a nucleotide (as a template) has a complementary effect, and no complementation occurs between itself. In magnesium ions, four single nucleotides Under the conditions of (dNTP), template DNA and primers, the PCR reaction is catalyzed by DNA polymerase, and the DNA is made by the instantaneous change of temperature. Denaturation, annealing and extension reactions occur to synthesize DNA fragments between two complementary sites. This reaction is repeated, making the first A loop-generated DNA fragment is amplified. After 25 to 30 cycles, the amplification factor reached 106. 3.1.3 A pair of oligonucleotides complementary to the DNA sequence of the two sides of the gene fragment to be amplified are synthesized by chemical method as PCR amplification Starting material. 3.1.4 In the detection of gene chips or other gene hybridization, chemical methods are used to synthesize oligonucleotide fragments of known specific DNA sequences. A segment, and a class of oligonucleotide products labeled with a label. 3.2 Abbreviations The following abbreviations apply to this standard. PCR polymerase chainreaction is abbreviated as PCR. Genechip genechip Gene chip. DNA deoxyribonucleicacid deoxyribonucleic acid.

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