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www.ChineseStandard.net Database: 189760 (25 Oct 2025)

SN/T 1525-2005 English PDF

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SN/T 1525-2005: Protocol of micro-complement fixation test for brucellosis
Status: Obsolete
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SN/T 1525-2005English399 Add to Cart 3 days [Need to translate] Protocol of micro-complement fixation test for brucellosis Obsolete SN/T 1525-2005

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Basic data

Standard ID SN/T 1525-2005 (SN/T1525-2005)
Description (Translated English) Protocol of micro-complement fixation test for brucellosis
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.020
Word Count Estimation 10,158
Date of Issue 2005-02-17
Date of Implementation 2005-07-01
Regulation (derived from) Industry standard filing Notice No. 4 of 2005 (No. 64 overall); National Quality recognize [2010] No. 582
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies: Brucellosis micro complement fixation test Operation technical requirements, This standard applies to: livestock brucellosis Serological diagnosis,

SN/T 1525-2005: Protocol of micro-complement fixation test for brucellosis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of micro-complement fixation test for brucellosis Book of the People's Republic of China Entry and Exit Inspection and Quarantine Brucellosis micro-complement binding test method Released on.2005-02-17 2005-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix C and Appendix D of this standard are normative appendices, and Appendix A and Appendix B are informative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic The main drafters of this standard. Wang Yuling, Cao Yuzheng, Zhu Shiqiang, Cai Guorui. This standard is the first industry standard for entry-exit inspection and quarantine. Brucellosis micro-complement binding test method

1 Scope

This standard specifies the operational technical requirements for the Brucellosis micro-complement binding test. This standard applies to the serological diagnosis of brucellosis in livestock.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682 analytical laboratory specifications and test methods

3 reagents and materials

3.1 Reagents 3.1.1 The distilled water used for the test is the tertiary water in GB/T 6682. 3.1.2 Diluent. normal saline. 3.1.3 Complement-binding antigen. Use according to the instructions and shake well before use. 3.1.4 Standard positive serum. Dissolve to the specified volume with distilled water before use. 3.1.5 Standard negative serum. Dissolve to the specified volume with distilled water before use. 3.1.6 Hemolysin. Dissolve to a specified volume with distilled water before use. 3.1.7 Complement. Dissolve to the specified volume with distilled water before use. 3.1.8 Whole blood of sheep. Choose healthy adult sheep, collect blood from sterile neck vein, add 1.2 parts of A's solution and add 1 part of blood, mix and store in 4°C refrigerator for 5 days. spare. 3.2 Materials 3.2.1 Glass test tube. 10mm × 75mm, round bottom, clean. 3.2.2 Test tube rack. 3.2.3 Micropipettes. 3.2.4 Adjustable continuous sampler. 3.2.5 96-well "U" type microplate. 3.2.6 96-well flat bottom ( ) microplate. 3.2.7 Horizontal rotor centrifuge. 3.2.8 ELISA plate reader. 3.3 Tested serum 3.3.1 Blood collection and separation Blood was collected and serum was separated by conventional methods. Serum must be fresh, free of protein flocs, no hemolysis, no spoilage. 3.3.2 Shipping and preservation Prevent the serum from freezing. If it cannot be sent to the laboratory within 3 days, the serum should be transported by cold storage. Serum should be stored in a refrigerator at 4 °C.

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