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SN/T 1505-2005: Inspection and identification of Radopholus (Cobb) Thorne
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Inspection and identification of Radopholus (Cobb) Thorne
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SN/T 1505-2005
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PDF similar to SN/T 1505-2005
Basic data | Standard ID | SN/T 1505-2005 (SN/T1505-2005) | | Description (Translated English) | Inspection and identification of Radopholus (Cobb) Thorne | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B16 | | Classification of International Standard | 65.020 | | Word Count Estimation | 46,474 | | Date of Issue | 2005-02-17 | | Date of Implementation | 2005-07-01 | | Regulation (derived from) | Industry standard filing Notice 2005 No. 4 (No. 64 overall) | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the perforation nematode (Radopholus Thorne, 1949), including former intends perforation nematode (Radopholoides de Guiran, 1967) quarantine and identification. This standard applies to plant propagation material and soil and cultivation medium perforations are quarantine and identification of nematodes. | SN/T 1505-2005: Inspection and identification of Radopholus (Cobb) Thorne---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Inspection and identification of Radopholus (Cobb) Thorne
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Perforation genus quarantine identification method
Released on.2005-02-17
2005-07-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Content
Foreword I
1 Scope 1
2 Principle 1
3 Instruments, utensils and medicines 1
4 On-site quarantine and laboratory nematode separation and identification 1
5 Morphological identification feature description 2
6 result judgment 3
7 Specimen and sample storage 3
Appendix A (Normative Appendix) Method for making perforated nematode slide specimens 4
Appendix C (informative) Morphological characteristics of perforated nematodes Figure 7
Appendix D (informative) Main host and geographical distribution of banana perforated nematodes 42
Foreword
Appendix A of this standard is a normative appendix, Appendix B, Appendix C, and Appendix D are informative appendices.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Xiamen Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Lin Qili, Chen Yong, Lin Shiming, Wang Hongyi, Zhang Tingzhou.
This standard is the first industry standard for entry-exit inspection and quarantine.
Perforation genus quarantine identification method
1 Scope
DeGuiran, 1967) Method of quarantine identification.
This standard is applicable to the quarantine and identification of perforated nematodes in plant propagation materials and soils and cultivation media.
2 Principle
(Tylenchida), Tylenchina, Tylenchoidea, and Pratylenchiidae. The genus
For the migration of endophytic nematodes for plant roots, the route of transmission is plant propagation material and soil and cultivation medium. According to mature male and female
The morphological characteristics of the worm were used to identify the genus of the genus.
3 Instruments, utensils and medicines
3.1 Instruments and appliances
Biological microscope, dissecting mirror, large tube centrifuge, incubator, mortar, centrifuge tube, funnel, funnel holder, rubber tube, water stop clip, separation tray,
Nematode filter paper, mesh screen (60 mesh, 400 mesh, 500 mesh), petri dishes, scissors, blades, balances, straws, beakers, slides, coverslips, alcohol lamps,
Pick a needle and so on.
3.2 Drugs
Alcohol, formalin, glacial acetic acid, glycerin, triethanolamine, polyvinyl alcohol, phenol, lactic acid, distilled water, neutral gum, and the like.
4 On-site quarantine and laboratory nematode separation and identification
4.1 On-site quarantine
4.1.1 Inspection
Check the documents (check the name, quantity, gimmick, place of origin and necessary quarantine documents, etc.) to match the goods.
Check the growth of plants (growth), pay attention to whether there are symptoms such as dwarfing, yellowing and wilt; focus on the roots of plants (roots, musts)
At the root, root of the eye) and at the petiole, observe whether there are red or black, burst and rot symptoms; cut open the root tissue with suspicious symptoms, check if there is
Typical symptoms such as discoloration, cavities, and tunnels. Collect the above suspected plant material and entrained soil, and send it to the laboratory for nematode separation test.
4.1.2 Sampling
In addition to the extraction of general samples, the focus should be on extracting samples with suspicious symptoms. Samples found along with on-site inspections
Samples of suspicious symptoms are packed in plastic bags and labeled; after being brought back to the laboratory, open the mouth of the bag, breathe properly, and place it at 20 °C~
25 ° C indoor to be tested.
4.2 Laboratory nematode separation test
4.2.1 Direct inspection
4.2.1.1 Anatomical examination
Remove the soil from the surface of the material, place it in a petri dish or a watch glass containing the appropriate amount of water, and use a dissecting needle to tear open the suspicious plant under a dissecting microscope.
Organize; pick the nematodes found and make temporary slides (see Appendix A) for inspection and identification.
4.2.1.2 Slice inspection
This method can be applied to the roots of herbs and succulent succulents.
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