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SN/T 1500-2004 English PDF

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SN/T 1500-2004: Determination of glycyrrhizic acid dipotassium in cosmetics. Liquid chromatography
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SN/T 1500-2004English199 Add to Cart 3 days [Need to translate] Determination of glycyrrhizic acid dipotassium in cosmetics. Liquid chromatography Valid SN/T 1500-2004

PDF similar to SN/T 1500-2004


Standard similar to SN/T 1500-2004

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Basic data

Standard ID SN/T 1500-2004 (SN/T1500-2004)
Description (Translated English) Determination of glycyrrhizic acid dipotassium in cosmetics. Liquid chromatography
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard Y42
Classification of International Standard 71.100.70
Word Count Estimation 6,662
Date of Issue 2004-11-17
Date of Implementation 2005-04-01
Regulation (derived from) 2005 Bulletin No. 3 industry standard
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the cosmetics Dipotassium licorice liquid chromatography detection methods. This standard applies to cosmetics Dipotassium licorice detection.

SN/T 1500-2004: Determination of glycyrrhizic acid dipotassium in cosmetics. Liquid chromatography


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Determination of glycyrrhizic acid dipotassium in cosmetics.Liquid chromatography Book of the People's Republic of China Entry and Exit Inspection and Quarantine Method for detecting dipotassium glycyrrhizinate in cosmetics Liquid chromatography Released on November 11,.2004 2005-04-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the China Academy of Inspection and Quarantine. The main drafters of this standard. Liu Song, Lin Yuanhui, Liu Juan. This standard is the first industry standard for entry-exit inspection and quarantine. Method for detecting dipotassium glycyrrhizinate in cosmetics Liquid chromatography

1 Scope

This standard specifies the liquid chromatographic method for the determination of dipotassium glycyrrhizinate in cosmetics. This standard applies to the detection of dipotassium glycyrrhizinate in cosmetics.

2 Principle

Dipotassium glycyrrhizinate in cosmetics was extracted with methanol, filtered, and separated by reversed-phase high performance liquid chromatography. According to its protection The time is fixed and the standard working curve method is used for quantification.

3 reagents and materials

Unless otherwise stated, the reagents used were of analytical grade and the water was secondary deionized water. 3.1 Methanol, chromatographically pure. 3.2 Acetonitrile, chromatographically pure. 3.3 Potassium dihydrogen phosphate, 0.02 mol/L. 3.4 Dipotassium glycyrrhizinate, purity ≥ 98%. 3.5 Dipotassium glycyrrhizinate standard stock solution (1g/L). Weigh 0.1g of dipotassium glycyrrhizinate, accurate to 0.1mg, in a 50mL beaker, add The amount of methanol was dissolved, and the solution was quantitatively transferred into a 100 mL volumetric flask, diluted with methanol to the mark, and mixed.

4 instruments

4.1 Liquid chromatograph with UV detector. 4.2 Microsampler, 10 μL. 4.3 Ultrasonic cleaner. 4.4 Centrifuge, 12000r/min. 4.5 Solvent filter and 0.45 μm filter membrane.

5 Analysis steps

5.1 Sample processing Weigh about 0.5g of cosmetic sample, accurate to 1mg, add 50mL of methanol in 50mL stoppered conical flask, and wash it by ultrasonic Ultrasonic vibration for 20 min in the device, quantitatively transfer the solution into a 50 mL volumetric flask, dilute to the mark with methanol, and mix. Take a part of the solution and put it away In the cardiac tube, the mixture was centrifuged at 12000 r/min for 10 min, and the supernatant after centrifugation was filtered through a 0.45 μm filter, and the filtrate was used for measurement. 5.2 Determination 5.2.1 Chromatographic conditions 5.2.1.1 Column. ODSC18 column (250 mm x 4.6 mm (inside diameter), 5 μm, or equivalent). 5.2.1.2 Mobile phase. acetonitrile. 0.02 mol/L potassium dihydrogen phosphate = 37. 63 (volume fraction). 5.2.1.3 Flow rate. 1.0 mL/min. 5.2.1.4 Detection wavelength. 254 nm. 5.2.1.5 Column temperature. room temperature.

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