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SN/T 1499-2004: Determination of kojic acid in cosmetics. Liquid chromatography
Status: Obsolete
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1499-2004 | English | 239 |
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Determination of kojic acid in cosmetics. Liquid chromatography
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SN/T 1499-2004
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Basic data | Standard ID | SN/T 1499-2004 (SN/T1499-2004) | | Description (Translated English) | Determination of kojic acid in cosmetics. Liquid chromatography | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 6,662 | | Date of Issue | 2004-11-17 | | Date of Implementation | 2005-04-01 | | Regulation (derived from) | 2005 Bulletin No. 3 industry standard | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the kojic acid in cosmetics by HPLC detection method. This standard applies to kojic acid in cosmetics testing. | SN/T 1499-2004: Determination of kojic acid in cosmetics. Liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of kojic acid in cosmetics.Liquid chromatography
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Method for detecting kojic acid in cosmetics
Liquid chromatography
Released on November 11,.2004
2005-04-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted by the China Academy of Inspection and Quarantine.
The main drafters of this standard. Liu Song, Lin Yuanhui, Chen Wei.
This standard is the first industry standard for entry-exit inspection and quarantine.
Method for detecting kojic acid in cosmetics
Liquid chromatography
1 Scope
This standard specifies the liquid chromatography method for the determination of kojic acid in cosmetics.
This standard applies to the detection of kojic acid in cosmetics.
2 Principle
The kojic acid in the cosmetics is extracted with water, filtered, and separated and measured by reversed-phase high performance liquid chromatography. According to its retention time
Qualitative, standard work curve method quantitative.
3 reagents and materials
Unless otherwise stated, the reagents used were of analytical grade and the water was secondary deionized water.
3.1 Methanol. chromatographically pure.
3.2 Potassium dihydrogen phosphate. 0.02mol/L
3.3 kojic acid. purity ≥ 99%;
3.4 Quacic acid standard stock solution (200mg/L). Weigh 0.02g kojic acid, accurate to 0.1mg, in a 50mL beaker, add appropriate amount of water to dissolve,
The solution was quantitatively transferred into a 100 mL volumetric flask, diluted to the mark with water, and mixed.
4 instruments
4.1 Liquid chromatograph with UV detector.
4.2 Microsampler, 10 μL.
4.3 Ultrasonic cleaner.
4.4 Centrifuge, 12000r/min.
4.5 Solvent filter and 0.45 μm filter membrane.
5 Determination steps
5.1 Sample processing
Weigh about 0.5g of cosmetic sample, accurate to 1mg, in a 50mL plugged conical flask, add 20mL of water, in the ultrasonic cleaner
The medium was shaken for 20 min, the solution was transferred to a 50 mL volumetric flask, diluted with water to the mark, and mixed. Take a part of the solution into the centrifuge tube,
After centrifugation at 12000 r/min for 10 min in a centrifuge, the supernatant after centrifugation was filtered through a 0.45 μm filter, and the filtrate was used for measurement.
5.2 Determination
5.2.1 Chromatographic conditions
5.2.1.1 Column. ODSC18 column [250 mm × 4.6 mm (inside diameter), 5 μm, or equivalent].
5.2.1.2 Mobile phase. methanol. 0.02 mol/L potassium dihydrogen phosphate = 18.82 (volume fraction).
5.2.1.3 Flow rate. 1.0mL/min;
5.2.1.4 Detection wavelength. 273 nm;
5.2.1.5 Column temperature. room temperature;
5.2.1.6 Injection volume. 10 μL.
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