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SN/T 1488-2004: Protocol of haemagglutination-inhibition test for viral haemorrhagic disease of rabbit
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Protocol of haemagglutination-inhibition test for viral haemorrhagic disease of rabbit
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SN/T 1488-2004
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Basic data | Standard ID | SN/T 1488-2004 (SN/T1488-2004) | | Description (Translated English) | Protocol of haemagglutination-inhibition test for viral haemorrhagic disease of rabbit | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 6,618 | | Date of Issue | 2004-11-17 | | Date of Implementation | 2005-04-01 | | Regulation (derived from) | 2005 Bulletin No. 3 industry standard | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the rabbit hemorrhagic disease virus hemagglutination inhibition test procedures. This standard applies to testing in rabbits immunized rabbit hemorrhagic disease virus and serum viral haemorrhagic disease antibodies. | SN/T 1488-2004: Protocol of haemagglutination-inhibition test for viral haemorrhagic disease of rabbit
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Protocol of haemagglutination-inhibition test for viral haemorrhagic disease of rabbit
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Rabbit viral hemorrhagic hemagglutination inhibition test procedure
Released on November 11,.2004
2005-04-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the Certification and Accreditation Administration of the People's Republic of China.
This standard was drafted. Jiangsu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Wang Shuiming, Peng Yuxin, Zhu Xueliang, Liu Xuehui.
This standard is the first industry standard for entry-exit inspection and quarantine.
Rabbit viral hemorrhagic hemagglutination inhibition test procedure
1 Scope
This standard specifies the operating procedures for hemagglutination inhibition test of rabbit viral hemorrhagic disease.
This standard is applicable to the detection of rabbit viral hemorrhagic disease virus in rabbits and rabbit viral hemorrhagic antibodies in serum.
2 Principle
Rabbit viral hemorrhagic virus can agglutinate human O-type red blood cells, but does not agglutinate or agglutinate red blood cells of other animals.
Obviously; after the rabbit viral hemorrhagic disease virus infects rabbits, rabbits will produce specific antibodies. This antibody and rabbit viral hemorrhagic disease virus
Thereafter, aggregation of the virus against human O-type red blood cells can be suppressed.
3 Materials and reagents
3.1 Instrument
Ordinary balance, analytical balance, common centrifuge, 96-well V-type blood coagulation plate, 25 μL adjustable pipette, incubator, micro-oscillator, etc.
3.2 Preparation of suspicious rabbits and normal rabbit liver suspension
The liver was cut separately, and the physiological saline was added 1.10, homogenized, centrifuged at 4000 r/min for 30 min, and the supernatant was taken for hemagglutination.
test.
3.3 saline
A physiological saline solution of 0.85% concentration was prepared.
See Appendix A for formulation.
3.5 Preparation of 1% human "O" type red blood cell suspension
Take fresh human “O” type red blood cells in a solution (preserved at 4 ° C for one week). Take human "O" type red blood cells at a time of 20 times
The washed red blood cells were mixed with saline, centrifuged at.2000 r/min for 15 min, the supernatant was discarded, and the washing was repeated four times. The washed red blood cells were mixed with physiological saline.
The 1% red blood cell suspension was set at 4 ° C for use and used up within 72 hours.
3.6 antigen, positive serum
It is supplied by the designated unit and used according to the instructions.
3.7 Treatment of tested serum
Blood samples were taken aseptically, serum was separated, and serum was inactivated at 56 ° C for 30 min.
4 method of operation
4.1 α-microhemagglutination inhibition test
4.1.1 Experimental steps
4.1.1.1 Each sample was made in the same 2 rows, and 25 μL of physiological saline was added from the first well to the 11th well.
4.1.1.2 The first and second wells were added with suspicious rabbit liver tissue suspension 25 μL.
4.1.1.3 Start from the second well, dilute to the 10th well with a 25μL pipette, and discard 25μL in the last well (the virus is diluted)
The degree is 10, 20, 40, 80, etc.).
4.1.1.4 The 12th hole is a normal rabbit liver suspension control.
4.1.1.5 In the second row, add 25 μL of positive serum to each well, shake well on a micro-oscillator, and place in a 37 ° C incubator for 10 min.
4.1.1.6 Add 25 μL of 1% human “O” type red blood cells to each well, shake immediately on the micro-vibrator, and set the refrigerator for 4 minutes at 45 °C.
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