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SN/T 1475-2004: Determination of arbutin in cosmetics liquid chromatography
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1475-2004 | English | 199 |
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Determination of arbutin in cosmetics liquid chromatography
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SN/T 1475-2004
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Basic data | Standard ID | SN/T 1475-2004 (SN/T1475-2004) | | Description (Translated English) | Determination of arbutin in cosmetics liquid chromatography | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | Y42 | | Classification of International Standard | 71.100.70 | | Word Count Estimation | 6,628 | | Date of Issue | 2004-11-17 | | Date of Implementation | 2005-04-01 | | Regulation (derived from) | 2005 Bulletin No. 3 industry standard | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the cosmetics arbutin liquid chromatography monitoring methods. This standard applies to cosmetics arbutin testing. | SN/T 1475-2004: Determination of arbutin in cosmetics liquid chromatography---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Determination of arbutin in cosmetics liquid chromatography
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
SN/T 1475-2004
Method for detecting arbutin in cosmetics
Liquid chromatography
Released on November 11,.2004
2005-04-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is an informative annex.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted by the China Academy of Inspection and Quarantine.
The main drafters of this standard. Liu Song, Liu Juan, Cai Tianpei.
This standard is the first industry standard for entry-exit inspection and quarantine.
SN/T 1475-2004
Method for the determination of arbutin in cosmetics by liquid chromatography
1 Scope
This standard specifies the liquid chromatographic method for the determination of arbutin in cosmetics.
This standard applies to the detection of arbutin in cosmetics.
2 Principle
The arbutin in the cosmetics is extracted with water, filtered, and separated and determined by reversed-phase high performance liquid chromatography. According to its retention
Qualitative, standard work curve method quantitative.
3 reagents and materials
Unless otherwise stated, the reagents used were of analytical grade and the water was secondary deionized water.
3.1 Methanol, chromatographically pure.
3.2 Arbutin, purity ≥ 99%.
3.3 Arbutin standard stock solution (1g/L). accurately weigh 0.1g arbutin, accurate to 0.1mg, in a 50mL beaker, add appropriate amount of water
Dissolve, quantitatively transfer the solution into a 100 mL volumetric flask, dilute to the mark with water, and mix.
4 instruments
4.1 Liquid chromatograph with UV detector.
4.2 Microsampler, 10 μL.
4.3 Ultrasonic cleaner.
4.4 Centrifuge, 12000r/min.
4.5 Solvent filter and 0.45 μm filter membrane.
5 Determination steps
5.1 Treatment of samples
Weigh about 0.5g of cosmetic sample, accurate to 1mg, add 50mL of water in a 50mL plugged conical flask, in an ultrasonic cleaner
The medium was shaken for 20 min, and the solution was transferred to a 50 mL volumetric flask, diluted with water to the mark, and mixed. Take a part of the solution into the centrifuge tube,
After centrifugation at 12000 r/min for 10 min in a centrifuge, the supernatant after centrifugation was filtered through a 0.45 μm filter, and the filtrate was used for measurement.
5.2 Determination
5.2.1 Chromatographic conditions
5.2.1.1 Column. ODSC18 column [250 mm × 4.6 mm (inside diameter), 5 μm, or equivalent].
5.2.1.2 Mobile phase. methanol. water = 15.85 (volume fraction).
5.2.1.3 Flow rate. 1.0 mL/min.
5.2.1.4 Detection wavelength. 280 nm.
5.2.1.5 Column temperature. room temperature.
5.2.1.6 Injection volume. 10 μL.
5.2.2 Standard working curve drawing
Transfer arbutin standard stock solution 0.20mL, 0.50mL, 1.00mL, 2.00mL, 3.00mL, 4.00mL, 5.00mL to one
In a series of 10 mL volumetric flasks, dilute to the mark with water and shake well. Take 10 μL of the solution into the liquid chromatograph and enter according to the chromatographic conditions (5.2.1).
SN/T 1475-2004
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