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SN/T 1468-2004: Protocol of haemagglutination-inhibition test for egg drop syndrome
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| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1468-2004 | English | 229 |
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Protocol of haemagglutination-inhibition test for egg drop syndrome
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SN/T 1468-2004
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Basic data | Standard ID | SN/T 1468-2004 (SN/T1468-2004) | | Description (Translated English) | Protocol of haemagglutination-inhibition test for egg drop syndrome | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 7,731 | | Date of Issue | 2004-11-17 | | Date of Implementation | 2005-04-01 | | Regulation (derived from) | 2005 Bulletin No. 3 industry standard | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the egg drop syndrome hemagglutination inhibition test methods. This standard applies to chicken egg drop syndrome virus infection or after vaccination serum antibodies, may also refer to other poultry quarantine. | SN/T 1468-2004: Protocol of haemagglutination-inhibition test for egg drop syndrome---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of haemagglutination-inhibition test for egg drop syndrome
People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard
Code of practice for hemagglutination inhibition test of chicken laying down syndrome
Released on November 11,.2004
2005-04-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Jiangsu Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Deng Juanxian, Ke Jiafa, Zhang Jingyou, Zhang Changyin, Zhang Yang.
This standard is the first industry standard for entry-exit inspection and quarantine.
Code of practice for hemagglutination inhibition test of chicken laying down syndrome
1 Scope
This standard specifies the test method for hemagglutination inhibition of laying down syndrome.
This standard is applicable to the detection of serum antibodies after chicken infection of egg drop syndrome virus or vaccination. Other poultry quarantine can also be referred to.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Materials and reagents
3.1 Equipment
3.1.1 Microplate. 96-well, V-bottomed, with a base angle of 90°.
3.1.2 Plastic blood collection tube. inner diameter 2mm, length 10cm.
3.1.3 syringe 2mL.
3.1.4 Plate. The diameter is 90mm.
3.2 Reagents
3.2.1 Reagent water should meet the requirements of GB/T 6682.
3.2.2 Antigen. supplied by the designated unit.
3.2.3 Standard positive serum. supplied by the designated unit.
3.2.4 phosphate buffered saline (PBS). pH 7.2 0.01 mol/L, the preparation method is shown in Appendix A.
3.2.5 Alsever's solution. See Appendix A for the preparation method.
3.2.6 1% chicken red blood cell suspension. take healthy adult chicken blood, mix with Alsever's solution in a ratio of 1.2, use 20 times the amount of physiological salt
The water was washed 3 times to 4 times, and each time was centrifuged at.2000 r/min for 5 minutes, and the last time was 10 minutes, and a 1% suspension was prepared with physiological saline. 4 ° C guarantee
Store the suspension and use it within 72 hours.
3.3 Tested serum
Puncture the lower vein of the chicken wing, use the plastic blood collection tube to introduce blood flow 6cm ~ 8cm long, burn one end, seal the mouth. Serum coagulation serum
After centrifugation at.2000 r/min for 5 min, the serum fraction was cut out and sealed for use. The chicks used a syringe to collect blood from the heart.
4 Hemagglutination inhibition test
4.1 Determination of antigen agglutination titer
4.1.1 The method for determining the agglutination titer of the antigen is shown in Table 1.
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