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SN/T 1465-2004 English PDF

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SN/T 1465-2004: Methods of quarantine and identification of Acidovorax avenae subsp. citrulli
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Basic data

Standard ID SN/T 1465-2004 (SN/T1465-2004)
Description (Translated English) Methods of quarantine and identification of Acidovorax avenae subsp. citrulli
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Classification of International Standard 65.020
Word Count Estimation 10,119
Date of Issue 2004-11-17
Date of Implementation 2005-04-01
Regulation (derived from) 2005 Bulletin No. 3 industry standard
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the phytosanitary bacterial fruit blotch of watermelon bacteria (Acidovorax avenae subsp. citrulli, referred Aac) identification methods. This standard applies to plant seeds of melons and watermelon bacterial fruit version bacteria identification.

SN/T 1465-2004: Methods of quarantine and identification of Acidovorax avenae subsp. citrulli

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Methods of quarantine and identification of Acidovorax avenae subsp.citrulli Book of the People's Republic of China Entry and Exit Inspection and Quarantine Method for quarantine and identification of watermelon bacterial spot disease Released on November 11,.2004 2005-04-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A and Appendix B of this standard are normative appendices, and Appendix C is an informative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted by the Xinjiang Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, and Nanjing Agricultural University participated in the drafting. The main drafters of this standard. Zhang Xianglin, Xue Guanghua, Hu Baishi, Zhang Wei, Fan Weigong, Yu Yongjie. This standard is the first industry standard for entry-exit inspection and quarantine. Method for quarantine and identification of watermelon bacterial spot disease

1 Scope

Identification method. This standard is applicable to the identification of bacterial fruit spot pathogens in watermelon seeds.

2 Principle

Bacterial Fruit Blotch is a dangerous disease on cucurbitaceous crops such as watermelon and melon. Webb1978) Wilems, Goor, Thielemans, Gilis, KerstersandDeLey.1992 VP], the genus prokaryote (Procaryotae), Gracilicutes, Scotobacteria, Pseudomonadaceae, hobby Characteristics such as sexual response are the basis of the quarantine identification method.

3 instruments and appliances

3.1 Biological microscope (with camera system). 3.2 Ultra-clean workbench. 3.3 Electronic balance (sensing 1/10000). 3.4 Light constant temperature incubator. 3.5 Constant temperature shaking incubator. 3.6 Low temperature refrigerator. 3.7 Autoclave. 3.8 Petri dish (diameter. 7.5 cm). 3.9 Constant temperature water bath (30 ° C ~ 95 ° C). 3.10 High-speed refrigerated centrifuge (18000r/min or more). 3.11 Small electric shredder. 3.12 PCR instrument. 3.13 Electrophoresis device (electrophoresis system, horizontal electrophoresis tank). 3.14 PCR tube (0.2 mL). 3.15 Centrifuge tubes (12 mL, 2 mL, 1.5 mL). 3.16 Micro-adjustable sample applicator (2μL, 10μL, 20μL, 100μL,.200μL, 1000μL). 3.17 Sampler tip (2μL～20μL, 20μL～200μL,.200μL～1000μL).

4 main reagents

Peptone, agar powder, glycerin, mercuric chloride, hydrochloric acid, sodium hypochlorite, dimethyl p-phenylenediamine hydrochloride, yeast extract, glucose, beef extract, phenol Red, berberine, carboxymethyl cellulose, boric acid, bright cresol blue, bromocresol purple, ethanol, cycloheximide, carbenicillin, ethylenediaminetetraacetic acid (EDTA), Tris, sodium dodecyl sulfate (SDS), liquid nitrogen, chloroform, isoamyl alcohol, isopropanol, agarose (for electrophoresis), ethidium bromide, T犪狇DNA polymerase, dipotassium hydrogen phosphate (K2HPO4), potassium dihydrogen phosphate (KH2PO4), disodium hydrogen phosphate (Na2HPO4·12H2O), ammonium dihydrogen phosphate (NH4H2PO4), magnesium sulfate (MgSO4·7H2O), calcium chloride (CaCl2·7H2O), chlorine

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