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SN/T 1420-2004: Protocol of diagnosis for whirling disease
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Protocol of diagnosis for whirling disease
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SN/T 1420-2004
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Basic data | Standard ID | SN/T 1420-2004 (SN/T1420-2004) | | Description (Translated English) | Protocol of diagnosis for whirling disease | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 8,836 | | Date of Issue | 2004-06-01 | | Date of Implementation | 2004-12-01 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the dizziness diagnosis procedures. This standard applies to export salmon trout dizziness disease quarantine. | SN/T 1420-2004: Protocol of diagnosis for whirling disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of diagnosis for whirling disease
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Dizziness diagnosis operation procedure
Released on June.2004
Implementation of.2004-12-01
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A, Appendix C and Appendix D of this standard are normative appendices, and Appendix B is an informative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Zhuhai Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Wu Xiaolun, Bo Qingru, Huang Jianzhen, Lai Ziping, Lan Naihong.
This standard is the first industry standard for entry-exit inspection and quarantine.
Dizziness diagnosis operation procedure
1 Scope
This standard specifies the diagnostic procedures for dizziness.
This standard is applicable to the inspection and quarantine of dizziness of imported and exported catfish.
2 materials
2.1 Equipment
Ordinary balance, analytical balance, general centrifuge, micro-oscillator, water bath, oscillating water bath, ordinary microscope, hemocytometer, pH test
Paper, mortar, scalpel, tweezers, beakers, slides, centrifuge tubes, adjustable pipettes, etc.
2.2 Reagents
Bleaching powder, 10% formalin, 1% malachite green, 70%, 80%, 90%, 95%, 100% alcohol, xylene, Giemsa staining solution (see attached
Record A), pepsin solution (0.5%) (see Appendix A), casein solution (0.1%) (see Appendix A), 0.1 mol/L sodium hydroxide, 10% cattle
Serum protein, phosphate buffered saline (PBS) (see Appendix A), methanol, acetone. All chemical reagents were of analytical grade.
3 Diagnosis of dizziness
3.1 clinical symptoms
The diseased fish is in a frantic rear-end campaign, especially when fed or frightened. In cold water conditions, the clinical manifestations of rotational motion are usually
It occurs 2 to 3 months after infection and can last up to one year. Diseased fish from 2 months to 8 months old with black at the back and tail of the trunk. faint
In the late stage of the erythema, the diseased fish sacral atrophy, the sputum is exposed, the mandible is deformed, the spine is curved, and the skull behind the eye is severely sunken.
It still carries pathogens after the year.
3.2 Parasitic examination
3.2.1 Pathogen morphology
(7.5μm～8μm), micro-ovate, flanked by small lentils; there are two pear-shaped capsules, two round embryo nucleus in the spore, a seamless ridge
Visible visible suture, no iodine foam (see Appendix B).
3.2.2 Sampling
3.2.2.1 Sampling principle
Select a clinically symptomatic fish for parasite examination. If it is asymptomatic, it can be randomly sampled according to the number of fish.
3.2.2.2 Number of samples
Take 5 to 10 tails for symptomatic fish for parasite examination, or for asymptomatic fish group according to Table C. 1 Conduct a spot check.
3.2.3 Smear inspection
3.2.3.1 Cut the head longitudinally and observe the presence or absence of cysts (milk-white, 1 mm to 2 mm in diameter) in the cartilage tissue of the ear region.
3.2.3.2 If there is a cyst, take a small cyst on a glass slide, crush it, and check it with a microscope from 450 to 600 times to see if there is a brain.
Sticky spores.
3.2.3.3 If no cysts are found, the cartilage tissue is cut from the otolith area and the contents are scraped for smear examination. Both the left and right ear stone areas need
an examination.
3.2.3.4 No spores were found in the above two tests, which can be checked according to 3.2.4.
3.2.4 Smear staining check
3.2.4.1 Cut the head of the suspect fish and cut it longitudinally, incubate in 45 ° C water for 1 min to 3 min, and move the loose meat and brain to a waste capacity.
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