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SN/T 1375-2004 English PDF

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SN/T 1375-2004: Methods for quarantine and identification of Pantoea stewartii (Smith) Mergaert et al.
Status: Valid

Standard ID	Contents [version]	USD	STEP2	[PDF] delivered in	Standard Title (Description)	Status	PDF
SN/T 1375-2004	English	319	Add to Cart	3 days [Need to translate]	Methods for quarantine and identification of Pantoea stewartii (Smith) Mergaert et al.	Valid	SN/T 1375-2004

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Basic data

Standard ID	SN/T 1375-2004 (SN/T1375-2004)
Description (Translated English)	Methods for quarantine and identification of Pantoea stewartii (Smith) Mergaert et al.
Sector / Industry	Commodity Inspection Standard (Recommended)
Classification of Chinese Standard	B16
Classification of International Standard	65.020
Word Count Estimation	10,112
Date of Issue	2004-06-01
Date of Implementation	2004-12-01
Issuing agency(ies)	General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary	This standard specifies the entry in the corn plant quarantine quarantine bacterial wilt pathogen various identification methods. This standard applies to entry of seed corn and other uses of bacterial wilt pathogen of maize quarantine and identification.

SN/T 1375-2004: Methods for quarantine and identification of Pantoea stewartii (Smith) Mergaert et al.

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Methods for quarantine and identification of Pantoea stewartii (Smith) Mergaert et al. People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Quarantine and identification method for bacterial wilt disease of maize Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A and Appendix B of this standard are normative appendices. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Tianjin Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Wei Yadong, Huang Guoming, Liu Yueting. This standard is the first industry standard for entry-exit inspection and quarantine.

Introduction

Stewart's Bacterial WiltofCorn is a devastating disease on corn. In.1992, the Ministry of Agriculture promulgated the "List of Imported Plant Quarantine for Sexually Transmitted Diseases, Insects and Weeds in the People's Republic of China", which is classified as a type of danger. Harmful creatures. In order to protect China's agricultural production, prevent the bacterial wilt of corn from being introduced into China, and do a good job in quarantine of imported plants, standardized and correct This standard is specially formulated to master the quarantine and identification methods of the pathogen. Quarantine and identification method for bacterial wilt disease of maize

1 Scope

This standard specifies the quarantine and identification methods for bacterial bacterial wilt pathogens in imported phytosanitary plants. This standard is applicable to the quarantine and identification of bacterial bacterial wilt pathogens in corn for introgression and other uses.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 4789.28-1994 Food hygiene microbiological examination Staining method, medium and reagents

3 Principle

3.1 Classification status of bacterial wilt pathogens in maize Dye, a prokaryotic organism, Procaryotae Bacterium, Bacillus, Gracilicutes, Enterobacteri- No flagella, blunt ends, solitary or short-chain. The bacterial blight of corn caused by the pathogen on corn is a typical dimension. Tube bundle disease, the disease is a species transmission, and the carrier seeds are the main factors for its long-distance transmission. 3.2 Principle of quarantine Enzyme-linked immunosorbent assay (ELISA) and immunoadsorption separation assays use serological techniques to have specific advantages, and conventional 4 instruments, equipment Small pulverizer, ultra-clean workbench, balance, constant temperature incubator, autoclave, biological microscope, microplate reader, plate washer, acidity meter, high Quick-frozen centrifuge, adjustable pipette, polystyrene plastic petri dish, etc. 5 drugs, reagents, culture media The drugs required for the test were of analytical grade. 5.1 Drugs Sodium carbonate (Na2CO3), sodium bicarbonate (NaHCO3), disodium hydrogen phosphate (Na2HPO4 · 12H2O), potassium dihydrogen phosphate (KH2PO4), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), Tween-20, dipotassium hydrogen phosphate (K2HPO4), magnesium sulfate (MgSO4·7H2O), hydrogen peroxide (H2O2), tetramethylbenzidine, dimethyl sulfoxide, peptone, sucrose (C12H22O11), ascorbic acid (C6H8O6), protein A (SPA), enzyme-labeled protein A (HRP-SPA), mercury (HgCl) or sodium hypochlorite (NaOCl), 95% ethanol (CH3CH2OH), and the like. 5.2 Reagents Gram staining solution, flagella staining solution. 5.3 medium Modified W's medium. See Appendix A for the production method.

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