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SN/T 1358.2-2005 English PDF

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SN/T 1358.2-2005: Equine infectious anemia agar gel immunodiffusion test for
Status: Obsolete
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SN/T 1358.2-2005English239 Add to Cart 2 days [Need to translate] Equine infectious anemia agar gel immunodiffusion test for Obsolete SN/T 1358.2-2005

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Basic data

Standard ID SN/T 1358.2-2005 (SN/T1358.2-2005)
Description (Translated English) Equine infectious anemia agar gel immunodiffusion test for
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 6,660
Date of Issue 2005-02-17
Date of Implementation 2005-07-01
Regulation (derived from) Industry standard filing Notice 2005 No. 4 (No. 64 overall)
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the equine infectious anemia agar gel immunodiffusion test procedures. This standard applies to equine infectious anemia antibodies for the detection and diagnosis.

SN/T 1358.2-2005: Equine infectious anemia agar gel immunodiffusion test for

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Equine infectious anemia agar gel immunodiffusion test for People's Republic of China Entry-Exit Inspection and Quarantine Industry Standard Equine infectious anemia agar gel immunization Diffusion test procedure Released on.2005-02-17 2005-07-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard is drafted by. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Yang Yuanjie, Zhu Laihua, Wu Hua, Han Guanglai. This standard is the first industry standard for entry-exit inspection and quarantine. Equine infectious anemia agar gel immunization Diffusion test procedure

1 Scope

This standard specifies the operating procedures for equine infectious anemia agar gel immunodiffusion test. This standard applies to the detection and diagnosis of antibody levels in equine infectious anemia.

2 Abbreviations

The following abbreviations apply to this standard. 2.1 AGIDTest. Agar immunodiffusion test. 2.2 EIA. Avian infectious anemia.

3 Principle

The molecular mass of antigens and antibodies is generally below 2 × 105 U, and the antigen and its specific antibody are in the gel from high concentration to low. When the concentration region diffuses, the resistance is small and is basically in the form of free diffusion. Due to the mass, structure, shape and charge of different antigen molecules Different, so the diffusion coefficient is different, and the diffusion speed in the gel is also different. When the antigen and the corresponding antibody are diffused, they meet in the gel. The antigen-antibody complex is formed, and if the ratio of the two is appropriate at the meeting, the largest complex is formed. Due to the large molecular mass of the complex, the particles increase Large, so no longer continue to spread and produce a precipitate, showing a linear or band shape, the formation of a linear or band-shaped "specific barrier" called immunity Precipitation line or immunoprecipitation zone, depending on whether there is such a precipitation zone between the antigen and the positive serum, whether precipitation occurs between the antigen and the test serum It is judged whether or not antibodies are present in the serum to be tested.

4 reagents and materials

4.1 EIAAGID antigen. provided by the designated unit. 4.2 Standard positive serum and standard negative serum. provided by the designated unit. 4.3 Tested serum. Serum was collected by blood sampling according to a conventional method. Store at 4 ° C in cold storage. 4.4 Disodium hydrogen phosphate (Na2HPO4·12H2O). analytically pure. 4.5 Potassium dihydrogen phosphate (KH2PO4). analytically pure. 4.6 Sodium chloride (NaCl). Analytically pure. 4.7 No ionized water. 4.8 Thimerosal. Analytical grade. 4.9 Agarose. Analytically pure.

5 instruments and equipment

5.1 Incubator. 5.2 Plate. Specification 90mm × 15mm, clean and sterilized. 5.3 Outer diameter 5.3mm puncher (or thin-walled metal tube). 5.4 Coordinate paper.

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