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SN/T 1350-2004 English PDF

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SN/T 1350-2004: Protocol of compliment fixation test for trypanosomiasis
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Basic data

Standard ID SN/T 1350-2004 (SN/T1350-2004)
Description (Translated English) Protocol of compliment fixation test for trypanosomiasis
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B41
Classification of International Standard 11.220
Word Count Estimation 8,886
Date of Issue 2004-06-01
Date of Implementation 2004-12-01
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the bovine trypanosomiasis supplies combined test methods. This standard applies to bovine trypanosomiasis antibodies.

SN/T 1350-2004: Protocol of compliment fixation test for trypanosomiasis

---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of compliment fixation test for trypanosomiasis Book of the People's Republic of China Entry and Exit Inspection and Quarantine Trypanosomiasis complement binding test method Released on June.2004 Implementation of.2004-12-01 People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is a normative appendix. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Shandong Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Meng Guang School, Xu Wei, Zhu Yongmei, and the selection. This standard is the first industry standard for entry-exit inspection and quarantine. Trypanosomiasis complement binding test method

1 Scope

This standard specifies the test method for complement fixation of bovine trypanosomiasis. This standard applies to the detection of antibodies to bovine trypanosomiasis.

2 test materials

2.1 Equipment 96-well “U” microplate, 10μL～100μL adjustable pipette, incubator, water bath, 15mL sharp bottom centrifuge tube, centrifuge, 12 mm x 75 mm test tube. 2.2 Reagents 2.2.1 Hemolysin. purchased from biopharmaceutical factory, the titer is not less than 1.1500. 2.2.2 Complement. Choose 3 to 4 adult male guinea pigs. After 6h-8h hunger strike, collect blood from the heart and separate fresh serum from the common Spare in the refrigerator. Freeze-dried complement can also be purchased. 2.2.3 Trypanosomiasis antigen, positive serum and negative serum. purchased from a biological product factory. 2.2.4 Preparation of sheep red blood cell suspension. see section A. Chapter 1. 2.2.5 Barbital Buffer (VBS for short). See section A. Chapter 2. 2.2.6 Preparation of hemolysis standard colorimetric wells. see section A. Chapter 3. 2.3 Serum treatment The tested serum, standard positive serum and standard negative serum were inactivated at 56 ° C for 30 min before use.

3 preliminary test

3.1 Determination of hemolysin titer 3.1.1 Prepare a 1.20 complement. 3.1.2 Hemolysin dilution. Hemolysin is made with VBS 1.500, 1.1000, 1.1500, 1.2000, 1.2500, 1.3000, 1.3500, 1.4000, 1.4500, 1.5000 dilution. 3.1.3 Hemolysin titer determination. 96-well "U"-type microplates were taken and added to each well according to the amount of each component in Table 1. Table 1 The unit of hemolysin titer is microliter ingredient Pipe number 1 2 3 4 5 6 7 8 9 10 Hemolysin Complement Red Blood Cells Hemolysin Dilution 500 × 1000 × 1500 ×.2000 × 2500 × 3000 × 3500 × 4000 × 4500 × 5000 × 1. 100 - - Hemolysin 25 25 25 25 25 25 25 25 25 25 25 - - VBS 50 50 50 50 50 50 50 50 50 50 75 75 100 complement 1.20 25 25 25 25 25 25 25 25 25 25 0 25 - 3% red Cell fluid 25 25 25 25 25 25 25 25 25 25 25 25 25

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