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SN/T 1316-2011: Quarantine protocol for bovine spongiform encephalopathy
Status: Valid SN/T 1316: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1316-2011 | English | 769 |
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6 days [Need to translate]
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Quarantine protocol for bovine spongiform encephalopathy
| Valid |
SN/T 1316-2011
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| SN/T 1316-2003 | English | 319 |
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3 days [Need to translate]
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Protocol of histopathological diagnosis for bovine spongiform encephalopathy
| Obsolete |
SN/T 1316-2003
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PDF similar to SN/T 1316-2011
Basic data | Standard ID | SN/T 1316-2011 (SN/T1316-2011) | | Description (Translated English) | Quarantine protocol for bovine spongiform encephalopathy | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 31,312 | | Date of Issue | 2011-05-31 | | Date of Implementation | 2011-12-01 | | Older Standard (superseded by this standard) | SN/T 1316-2003 | | Quoted Standard | GB/T 6682; GB 19489 | | Regulation (derived from) | National-Quality-Inspection-accreditation (2011) 291 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the sample of bovine spongiform encephalopathy, histopathological examination methods, immunohistochemistry, protein immunoblotting, ELISA test methods, technical requirements immunochromatographic strip detection methods, such as detection methods and operations specification. Histopathological examination method in which an auxiliary method of immunohistochemistry. SAF immunohistochemistry and Western blot diagnostic method for the diagnosis. Western blot method, ELISA method and immunochromatographic test strip detection method for screening methods. This standard applies to the diagnosis of bovine spongiform encephalopathy, can also be used for the diagnosis of transmissible spongiform encephalopathies other animals. | SN/T 1316-2011: Quarantine protocol for bovine spongiform encephalopathy---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Quarantine protocol for bovine spongiform encephalopathy
People's Republic of China Entry-Exit Inspection and Quarantine Standards
Instead of the SN/T 1316-2003
BSE Technical Specifications quarantine
Issued on. 2011-05-31
2011-12-01 implementation
People's Republic of China
The State Administration of Quality Supervision, Inspection and Quarantine released
Foreword
This standard was drafted in accordance with GB/T 1.1-2009 given rules.
Instead of the standard SN/T 1316-2003 "bovine spongiform encephalopathy histopathological examination methods."
This standard compared with SN/T 1316-2003, major technical changes are as follows.
--- Increased BSE sampling;
--- Increasing the Western blot method;
--- Increased enzyme linked immunosorbent assay method (ELISA);
--- Increased immunochromatographic strip for detection methods.
The standard reference of the International Organization for Animal Health (OIE) of "diagnostic tests and vaccines Standards Manual" (2008 Edition) "ManualofDiag-
nosticTestsandVaccinesforTerrestrialAnimals ") in Chapter 2.4.6" BSE diagnostic techniques. "
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
This standard was drafted. People's Republic of China Beijing Exit Inspection and Quarantine drafted.
The main drafters of this standard. Ma Guiping, Liyan Xin, Li Bingling, Liu Quanguo, Shixi Ju, Liu Xuhui.
This standard replaces the standards previously issued as follows.
--- SN/T 1316-2003.
BSE Technical Specifications quarantine
1 Scope
This standard specifies the sampling of bovine spongiform encephalopathy histopathological examination method, immunohistochemistry, Western blot method,
Technical requirements ELISA test methods, immunochromatographic strip detection methods and other detection methods and practices. Wherein Histopathology
Examination method immunohistochemistry helper methods, immunohistochemistry and Western blot SAF diagnosed as confirmed side
Method, Western blot method, ELISA method and immunochromatographic strip for detection method for the screening method.
This standard applies to the diagnosis of bovine spongiform encephalopathy, also be used for the diagnosis of transmissible spongiform encephalopathies other animals.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682 Analysis
GB 19489 General requirements for laboratory biosafety
3 clinical diagnosis
3.1 Bovine Spongiform Encephalopathy (BSE) average incubation period of 4 years to 5 years. Cows clinical manifestations of mental disorders, movement disorders and sensory
obstacle.
3.2 psychiatric disorders. mainly as anxiety, fear, rage, etc., or when someone close to persecute them when often appear aggressive behavior.
3.3 movement disorders. mainly as ataxia, tremor or fall. Cow gait was "goose step" shaped limbs hyperextension, hind limb motor loss
Tone, tremors and falls, paralysis, paresis; sometimes fell hard to stand.
3.4 sensory dysfunction. The most common is a touch over-sensitive sound and light, this is a very important clinical BSE cow diagnostic features. Hand
Touch or touch and pressure with a blunt neck rib cattle, cows will be extremely nervous trembling, light touch with a broom heels, there will be tension kicks reaction; disease
Cattle knocker metal instrument sounds, there will be shocked and trembling reaction; cows in a dark environment, the sudden opening of the lights appeared frightened
And shivering reaction.
4 Laboratory diagnosis
4.1 Sampling and sample preparation methods
4.1.1 Materials
4.1.1.1 subject tissue
Be seized bovine brain.
4.1.1.2 Reagents
10% neutral formalin solution (see Appendix A); disinfectant. 2% sodium hypochlorite, 2mol/L sodium hydroxide; 98% to 99% formic acid; no
Aqueous ethanol; 95% ethanol; 70% ethanol solution; xylene; paraffin wax (melting point 54 ℃ ~ 56 ℃, 56 ℃ ~ 58 ℃, 58 ℃ ~ 60 ℃).
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