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SN/T 1315-2010: Protocol of quarantine for enzootic bovine leukosis
Status: Valid SN/T 1315: Evolution and historical versions
| Standard ID | Contents [version] | USD | STEP2 | [PDF] delivered in | Standard Title (Description) | Status | PDF |
| SN/T 1315-2010 | English | 369 |
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Protocol of quarantine for enzootic bovine leukosis
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SN/T 1315-2010
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| SN/T 1315-2003 | English | 319 |
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Protocol of agar gel immunodiffusion test for enzootic bovine leukosis
| Obsolete |
SN/T 1315-2003
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PDF similar to SN/T 1315-2010
Basic data | Standard ID | SN/T 1315-2010 (SN/T1315-2010) | | Description (Translated English) | Protocol of quarantine for enzootic bovine leukosis | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Classification of International Standard | 11.220 | | Word Count Estimation | 14,135 | | Date of Issue | 2010-01-10 | | Date of Implementation | 2010-07-16 | | Older Standard (superseded by this standard) | SN/T 1315-2003 | | Quoted Standard | SN/T 1917 | | Regulation (derived from) | National Quality Inspection [2010] No. 13 | | Issuing agency(ies) | General Administration of Customs | | Summary | This standard specifies the enzootic bovine leukemia clinical diagnosis and laboratory diagnosis of operational requirements. This standard applies to enzootic bovine leukemia quarantine. | SN/T 1315-2003: Protocol of agar gel immunodiffusion test for enzootic bovine leukosis---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of agar gel immunodiffusion test for enzootic bovine leukosis
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Bovine local epidemic leukemia agar immunity
Diffusion test procedure
Released on.2003-08-18
2004-02-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Chen Shisong, Cui Xiangdong, Zhao Zenglian.
This standard is the industry standard for inspection and quarantine issued for the first time.
Bovine local epidemic leukemia agar immunity
Diffusion test procedure
1 Scope
This standard specifies the operating procedures for the adenine immunodiffusion test for bovine endemic leukemia.
This standard applies to the detection of antibodies to bovine endemic leukemia virus.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682-1992 Analytical laboratory water specifications and test methods
3 Abbreviations
The following abbreviations apply to this standard.
3.1
Agar immunodiffusion test.
3.2
EBL
Bovine local epidemic leukemia.
4 Principle
Antigen and specific antibodies are less resistant to diffusion from a high concentration region to a low concentration region in the gel, and are substantially free to diffuse.
form. Due to the different molecular mass, structure, shape and charge of different antigen molecules, the diffusion coefficient is different and the expansion in the gel
The speed of dispersion is also different. When the antigen and the corresponding antibody are diffused, they meet in the gel to form an antigen-antibody complex, if the two are at the meeting
When appropriate, the largest composite is formed. As the molecular weight of the composite increases, the particles increase, so that it does not continue to diffuse and form a precipitate.
It is linear, called immunoprecipitation line or immunoprecipitation zone. AGIDTest is based on the precipitation line between the antigen and the positive serum.
Determine whether specific antibodies are present in the serum to be tested.
5 reagents and equipment
5.1 antigen
The embryonic kidney cell (FLK) culture medium infected with EBL virus is prepared by extraction and concentration, and is mainly composed of viral envelope glycoprotein (gp).
And the mixed antigen containing nucleus pulposus protein (p), the viral envelope glycoprotein titer should not be less than four units, between the test and the standard positive serum
A clear sedimentation line must appear within 24 hours, and no sedimentation line should appear between the standard negative serum. The antigen is supplied as a lyophilized product,
Dissolve in water and return to the original volume.
5.2 Standard positive serum
It can produce a clear and dense sediment line (gp antibody) within 24h with the standard antigen, and the end contacts the adjacent pores on both sides.
edge.
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