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US$239.00 · In stock Delivery: <= 3 days. True-PDF full-copy in English will be manually translated and delivered via email. SN/T 1314-2003: Protocol of counter immunoelectrophoresis for mink aleutian disease Status: Obsolete
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Protocol of counter immunoelectrophoresis for mink aleutian disease
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SN/T 1314-2003
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Basic data | Standard ID | SN/T 1314-2003 (SN/T1314-2003) | | Description (Translated English) | Protocol of counter immunoelectrophoresis for mink aleutian disease | | Sector / Industry | Commodity Inspection Standard (Recommended) | | Classification of Chinese Standard | B41 | | Word Count Estimation | 6,687 | | Date of Issue | 2003-08-18 | | Date of Implementation | 2004-02-01 | | Quoted Standard | GB/T 6682 | | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China | | Summary | This standard specifies the Aleutian mink disease counter immuno electrophoresis assay procedures. This standard applies to a counter immuno electrophoresis to detect enter, exit Aleutian mink disease antibodies. |
SN/T 1314-2003: Protocol of counter immunoelectrophoresis for mink aleutian disease---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of counter immunoelectrophoresis for mink aleutian disease
Book of the People's Republic of China Entry and Exit Inspection and Quarantine
Oyster Aleutian disease convective immunoelectrophoresis operation procedure
Released on.2003-08-18
2004-02-01 implementation
People's Republic
The General Administration of Quality Supervision, Inspection and Quarantine issued
Foreword
This standard electrophoresis operation method refers to the quarantine method of the Animal Quarantine Operation Regulations (1988 edition) of the Ministry of Agriculture, and has developed sample loading.
Pre-processing method.
Appendix A of this standard is a normative appendix.
This standard is proposed and managed by the National Certification and Accreditation Administration.
This standard was drafted. Beijing Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China.
The main drafters of this standard. Lai Pingan, Zhang Hexiao, Liu Yanhua.
This standard is the industry standard for inspection and quarantine issued for the first time.
Oyster Aleutian disease convective immunoelectrophoresis operation procedure
1 Scope
This standard specifies the operating procedures for convective immunoelectrophoresis testing of leech Aleutian disease.
This standard is applicable to the detection of influent Aleutian disease antibodies by convection immunoelectrophoresis.
2 Normative references
The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all
Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study
Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard.
GB/T 6682 Analytical laboratory water specifications and test methods
3 Material preparation
3.1 Instruments and equipment
3mm thick 100mm × 45mm glass plate; voltage 0V ~ 300V, current 0mA ~ 50mA electrophoresis instrument; width 100mm
Electrophoresis tank; 3mm diameter puncher; 100μL micro-adjustable pipette and matching tip; plastic capillary blood collection tube.
Barbital sodium 10.31g
Tris-hydroxymethylaminomethane 6.005g
Sodium azide 0.2g
Secondary water (in accordance with GB/T 6682) 1000mL
After adding the appropriate amount of secondary water (according to GB/T 6682), dissolve the pH value to 8.5-8.6 with hydrochloric acid, and finally use the second grade.
Water (in accordance with GB/T 6682) to a volume of 1000mL.
3.3 agarose
Analytically pure agarose for domestic or imported electrophoresis.
3.4 antigen
It was prepared from a cat kidney passage cell culture of the Aleutian virus international standard strain Utah-1. Provided by the designated unit.
3.5 serum
3.5.1 Positive serum. provided by the designated unit.
3.5.2 Negative serum. provided by the designated unit.
3.5.3 Serum to be tested. Blood is collected from the tip of the toe with a plastic capillary tube. The two ends of the tube are sealed and sealed, placed in an environment of 20 ° C to 30 ° C for 1 h, and then placed.
4 ° C refrigerator 18h ~ 20h, precipitated serum, add 0.5mL or 1.5mL centrifuge tube, centrifuge at 3000r/min for 5min, take the supernatant
spare.
4 operating procedures
4.1 Boarding
Take 3mm thick 100mm × 45mm horizontal scratch-free glass plate, wash the oil, put it on the horizontal table, use 10mL
The pipette draws 0.8% agarose buffer which has been dissolved and cooled to about 70 ° C, and is evenly spread on a glass plate with a thickness of 3 mm.
When the glass plate is surrounded by a baffle, it will be used after cooling and solidification, and it will be used up on the same day.
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