SN/T 1204-2016 (SN/T1204-2016, SNT 1204-2016, SNT1204-2016)
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SN/T 1204-2016 | English | 509 |
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Protocol of the real-time PCR method for detecting genetically modified plants and their derived products
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SN/T 1204-2016
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SN/T 1204-2003 | English | 90 |
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Protocol of the real-time PCR for detecting genetically modified plants and their derived products
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SN/T 1204-2003
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Standards related to: SN/T 1204-2016
Standard ID | SN/T 1204-2016 (SN/T1204-2016) | Description (Translated English) | Protocol of the real-time PCR method for detecting genetically modified plants and their derived products | Sector / Industry | Commodity Inspection Standard (Recommended) | Classification of Chinese Standard | B16 | Word Count Estimation | 22,254 | Date of Issue | 2016-06-28 | Date of Implementation | 2017-02-01 | Older Standard (superseded by this standard) | SN/T 1204-2003 | Drafting Organization | China Academy of Inspection and Quarantine, People's Republic of China Exit Inspection and Quarantine, People's Republic of China Shanghai Exit Inspection and Quarantine, Shenzhen Inspection and Quarantine Science Institute | Administrative Organization | Certification and Accreditation Administration of the People's Republic of China | Regulation (derived from) | State-Quality-Inspection-Accredidation [2016] No.318 | Proposing organization | Certification and Accreditation Administration of the People's Republic of China | Issuing agency(ies) | General Administration of Quality Supervision, Inspection and Quarantine of People's Republic of China |
SN/T 1204-2016
Protocol of the real-time PCR method for detecting genetically modified plants and their derived products
People's Republic of China Exit Inspection and Quarantine standards
Replacing SN/T 1204-2003
Transgenic components in plants and their processed products in real time
Fluorescent PCR qualitative test method
2016-06-28 release
2017-02-01 Implementation
People's Republic of China
General Administration of Quality Supervision, Inspection and Quarantine released
Exit Inspection and Quarantine of People's Republic of China
Industry Standard
Transgenic components in plants and their processed products in real time
Fluorescent PCR qualitative test method
China Standard Press
First edition in October.2017
Foreword
This standard was drafted in accordance with the rules given in GB/T 1.1-2009.
This standard replaces SN/T 1204-2003 "plants and their processed products GMO qualitative and quantitative real-time fluorescent PCR test."
This standard compared with SN/T 1204-2003, the main changes are as follows.
--- Increased plant endogenous gene detection methods.
--- Increasing the screening of genetically modified plants detection methods.
--- Increased soybean, corn, rape, cotton, rice, potatoes, flax, sugar beet and other crops line-specific detection methods.
This standard is proposed and managed by the National Certification and Accreditation Administration Committee.
The main drafting units of this standard.China Inspection and Quarantine Science Institute, People's Republic of China Shandong Exit Inspection and Quarantine, Chinese
People's Republic of China Shanghai Exit Inspection and Quarantine, Shenzhen Inspection and Quarantine Science Institute.
The main drafters of this standard. Huang Xin, Zhu Shuifang, Gao Hongwei, Li think, Pan Liangwen, Chen Hongjun, Chen Zhinan.
This standard replaces the standards previously issued as.
--- SN/T 1204-2003.
Transgenic components in plants and their processed products in real time
Fluorescent PCR qualitative test method
1 Scope
This standard specifies the plant and its processing products GMO screening and line identification of real-time PCR detection method.
This standard applies to soybeans, corn, rapeseed, rice, cotton, potatoes, flax, beet, alfalfa, tomato, papaya, apple, chicory, scissors
Stem, tobacco, plum, melon, wheat, eggplant and eucalyptus transgenic screening test also applies to soybeans, corn, rapeseed, rice, cotton,
Line-specific real-time PCR detection of crops such as potato, flax and beet.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB/T 19495.2 Genetically Modified Products Testing Laboratory technical requirements
GB/T 19495.3 Genetically modified products for nucleic acid extraction and purification methods
GB/T 19495.7 Genetically Modified Products - Sampling and Sample Preparation Methods
GB/T 27403 laboratory quality control specification food molecular biology test
3 Terms, definitions and abbreviations
3.1 Terms and definitions
The following terms and definitions apply to this document.
3.1.1
Transgenic transgene
The species do not have their own, derived from other species of functional DNA sequences, through bio-engineering technology, make it into the species
Line expression, so that the species access to new breed characteristics.
3.1.2
Real-time fluorescence PCR real-timepolymerasechainreaction
Real-time fluorescence polymerase chain reaction. Refers to the polymerase chain reaction system by adding fluorescent groups, the use of fluorescence signal accumulation in real time
Monitoring the entire PCR process, the strength of fluorescent signal directly reflects the number of templates.
3.1.3
Endogenous gene endogenous gene
A copy number constant in the test species that does not show allelic changes. This gene can be used to determine species specificity.
3.1.4
Exogenous gene exogenous gene
Other biogenic genes that are transferred using bioengineering techniques enable the biological species to express new biological traits.
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