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SN/T 1204-2016 English PDF

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SN/T 1204-2016: Protocol of the real-time PCR method for detecting genetically modified plants and their derived products
Status: Valid

SN/T 1204: Evolution and historical versions

Standard IDContents [version]USDSTEP2[PDF] delivered inStandard Title (Description)StatusPDF
SN/T 1204-2016English509 Add to Cart 5 days [Need to translate] Protocol of the real-time PCR method for detecting genetically modified plants and their derived products Valid SN/T 1204-2016
SN/T 1204-2003English90 Add to Cart 0--9 seconds. Auto-delivery Protocol of the real-time PCR for detecting genetically modified plants and their derived products Obsolete SN/T 1204-2003

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Basic data

Standard ID SN/T 1204-2016 (SN/T1204-2016)
Description (Translated English) Protocol of the real-time PCR method for detecting genetically modified plants and their derived products
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B16
Word Count Estimation 22,218
Date of Issue 2016-06-28
Date of Implementation 2017-02-01
Older Standard (superseded by this standard) SN/T 1204-2003
Regulation (derived from) State-Quality-Inspection-Accredidation [2016] No.318
Issuing agency(ies) General Administration of Customs

SN/T 1204-2016: Protocol of the real-time PCR method for detecting genetically modified plants and their derived products


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of the real-time PCR method for detecting genetically modified plants and their derived products People's Republic of China Exit Inspection and Quarantine standards Replacing SN/T 1204-2003 Transgenic components in plants and their processed products in real time Fluorescent PCR qualitative test method 2016-06-28 release 2017-02-01 Implementation People's Republic of China General Administration of Quality Supervision, Inspection and Quarantine released Exit Inspection and Quarantine of People's Republic of China Industry Standard Transgenic components in plants and their processed products in real time Fluorescent PCR qualitative test method China Standard Press First edition in October.2017

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009. This standard replaces SN/T 1204-2003 "plants and their processed products GMO qualitative and quantitative real-time fluorescent PCR test." This standard compared with SN/T 1204-2003, the main changes are as follows. --- Increased plant endogenous gene detection methods. --- Increasing the screening of genetically modified plants detection methods. --- Increased soybean, corn, rape, cotton, rice, potatoes, flax, sugar beet and other crops line-specific detection methods. This standard is proposed and managed by the National Certification and Accreditation Administration Committee. The main drafting units of this standard.China Inspection and Quarantine Science Institute, People's Republic of China Shandong Exit Inspection and Quarantine, Chinese People's Republic of China Shanghai Exit Inspection and Quarantine, Shenzhen Inspection and Quarantine Science Institute. The main drafters of this standard. Huang Xin, Zhu Shuifang, Gao Hongwei, Li think, Pan Liangwen, Chen Hongjun, Chen Zhinan. This standard replaces the standards previously issued as. --- SN/T 1204-2003. Transgenic components in plants and their processed products in real time Fluorescent PCR qualitative test method

1 Scope

This standard specifies the plant and its processing products GMO screening and line identification of real-time PCR detection method. This standard applies to soybeans, corn, rapeseed, rice, cotton, potatoes, flax, beet, alfalfa, tomato, papaya, apple, chicory, scissors Stem, tobacco, plum, melon, wheat, eggplant and eucalyptus transgenic screening test also applies to soybeans, corn, rapeseed, rice, cotton, Line-specific real-time PCR detection of crops such as potato, flax and beet.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article Pieces. For undated references, the latest edition (including all amendments) applies to this document. GB/T 6682 analytical laboratory water specifications and test methods GB/T 19495.2 Genetically Modified Products Testing Laboratory technical requirements GB/T 19495.3 Genetically modified products for nucleic acid extraction and purification methods GB/T 19495.7 Genetically Modified Products - Sampling and Sample Preparation Methods GB/T 27403 laboratory quality control specification food molecular biology test 3 Terms, definitions and abbreviations 3.1 Terms and definitions The following terms and definitions apply to this document. 3.1.1 Transgenic transgene The species do not have their own, derived from other species of functional DNA sequences, through bio-engineering technology, make it into the species Line expression, so that the species access to new breed characteristics. 3.1.2 Real-time fluorescence PCR real-timepolymerasechainreaction Real-time fluorescence polymerase chain reaction. Refers to the polymerase chain reaction system by adding fluorescent groups, the use of fluorescence signal accumulation in real time Monitoring the entire PCR process, the strength of fluorescent signal directly reflects the number of templates. 3.1.3 Endogenous gene endogenous gene A copy number constant in the test species that does not show allelic changes. This gene can be used to determine species specificity. 3.1.4 Exogenous gene exogenous gene Other biogenic genes that are transferred using bioengineering techniques enable the biological species to express new biological traits.

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