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SN/T 1200-2003 English PDF

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SN/T 1200-2003: Protocol of qualitative PCR for the detection genetically modified components in tobacco
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PDF similar to SN/T 1200-2003


Standard similar to SN/T 1200-2003

GB/T 12729.2   SN/T 0803.6   GB 2635   SN/T 1146.2   

Basic data

Standard ID SN/T 1200-2003 (SN/T1200-2003)
Description (Translated English) Protocol of qualitative PCR for the detection genetically modified components in tobacco
Sector / Industry Commodity Inspection Standard (Recommended)
Classification of Chinese Standard B35
Word Count Estimation 10,146
Date of Issue 2003-03-17
Date of Implementation 2003-09-01
Issuing agency(ies) General Administration of Quality Supervision, Inspection and Quarantine of the People Republic of China
Summary This standard specifies the genetically modified tobacco Qualitative PCR detection methods. This standard applies to fresh and dried leaves, seeds and genetically modified tobacco and other qualitative detection and identification.

SN/T 1200-2003: Protocol of qualitative PCR for the detection genetically modified components in tobacco


---This is a DRAFT version for illustration, not a final translation. Full copy of true-PDF in English version (including equations, symbols, images, flow-chart, tables, and figures etc.) will be manually/carefully translated upon your order.
Protocol of qualitative PCR for the detection genetically modified components in tobacco Book of the People's Republic of China Entry and Exit Inspection and Quarantine Qualitative PCR detection method for transgenic components in tobacco Released on.2003-03-17 2003-09-01 implementation People's Republic The General Administration of Quality Supervision, Inspection and Quarantine issued

Foreword

Appendix A of this standard is an informative annex. This standard is proposed and managed by the National Certification and Accreditation Administration. This standard was drafted. Yunnan Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China. The main drafters of this standard. Xu Zizhong, Jia Jianjun, Zhou Xiaoli, Dan Youmin, Zhou Libing. This standard is the industry standard for inspection and quarantine issued for the first time. Qualitative PCR detection method for transgenic components in tobacco

1 Scope

This standard specifies the qualitative PCR detection method for transgenic components in tobacco. This standard is applicable to the qualitative detection and identification of genetically modified components such as fresh and dried tobacco leaves, seeds and flue-cured tobacco.

2 Normative references

The terms in the following documents become the terms of this standard by reference to this standard. All dated references, followed by all Modifications (not including errata content) or revisions do not apply to this standard, however, parties to agreements based on this standard are encouraged to study Is it possible to use the latest version of these files? For undated references, the latest edition applies to this standard. GB/T 6682 Analytical laboratory water specifications and test methods SN/T 1193 Genetic Testing Laboratory Technical Requirements SN/T 1194 Detection and sampling method for genetically modified components of plants and their products Qualitative test method for real-time fluorescent PCR of genetically modified components in SN/T 1204 plants and their processed products 3 Terms, definitions and abbreviations The following terms, definitions and abbreviations apply to this standard. 3.1 Terms and definitions 3.1.1 A functional DNA sequence derived from other species that is not native to the species, and which is introduced into the species through various means of introduction. Line expression so that the species acquires new cultivar characteristics. 3.1.2 The template gene sequence is first denatured to a single strand by high temperature, and is set according to the template sequence under the action of DNA polymerase and suitable reaction conditions. The two primers are respectively annealed to the corresponding complementary sequence on the two strands of the template DNA, and then combined with each other, followed by DNA polymerization. Under the action of enzymes, four kinds of deoxyribonucleic acids (dNTPs) are used as substrates to extend the primers, and then the denaturation, annealing and extension are repeated. In one cycle, the gene fragment to be amplified is increased in geometric multiples. 3.2 Abbreviations 3.2.1 UNG. uracil DNAglycosylase, uracil DNA-glycosylase. 3.2.2 EDTA. ethylenediaminetetraacetic acid, ethylenediaminetetraacetic acid. 3.2.3 CTAB. Cetyltriethylammonium bromide. 3.2.4 TMV-CP. tobaccomosiacviruscoatprotain, tobacco mosaic virus coat protein gene. 3.2.5 CMV-CP. cucumbermosiacviruscoatprotain, cucumber mosaic virus coat protein gene. 3.2.6 TMV-54KD. tobaccomosiacvirus 54KDgene, tobacco mosaic virus 54KD protein gene. 3.2.8 PVY-CP. potatovirus Ycoatprotain, potato virus Y coat protein gene. 3.2.9 TBE. Tris base, boric acid, EDTA buffer. 3.2.10 NPT-II. neomycin-3'-phosphotransferasegene, neomycin-3'-phosphotransferase gene. 3.2.11 NOS. terminatorofnopalinesynthasegene, the nopaline synthase gene terminator. 3.2.12 CaMV35S. 35Spromoterfromcauliflowermosaicvirus, 35S from cauliflower mosaic virus

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